Evaluation of antagonistic ability against by colletotrichum fructicola CL5 causing anthracnose disease on chilli by β-1,3-glucanase producing bacillus spp.
Vol. 133 No. 3A (2024), Original Article
Vol. 133 No. 3A (2024)

Evaluation of antagonistic ability against by colletotrichum fructicola CL5 causing anthracnose disease on chilli by β-1,3-glucanase producing bacillus spp.

Original Article
https://doi.org/10.26459/hueunijard.v133i3A.7379
Published 2024-03-29
Nguyen Thi Thanh Huyen
Institute of Biotechnology, Hue University, Nguyen Đinh Tu St., Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam
Nguyen Duc Huy
Institute of Biotechnology, Hue University, Nguyen Dinh Tu St., Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam
Nguyen Bao Hung
Institute of Biotechnology, Hue University, Nguyen Dinh Tu St., Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam
Le Thi Nhat Anh
Institute of Biotechnology, Hue University, Nguyen Dinh Tu St., Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam
Nguyen Thi Minh Nga
Institute of Biotechnology, Hue University, Nguyen Dinh Tu St., Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam
Tran Quoc Dung
University of Education, Hue University, 32 Le Loi St., Hue, Vietnam
PDF (Vietnamese)

Keywords

β-1,3-glucanase
Bacillus
Colletotrichum fructicola
biocontrol β-1,3-glucanase
Bacillus
Colletotrichum fructicola,
đối kháng sinh học

Abstract

β-1,3-glucanase is a crucial enzyme for the development of plants. It is involved in the maturation of seeds, floral development, and cell division. Furthermore, β-1,3-glucanase prevents the spreading of diseases by fungal pathogens to plants. This study chose two bacterial strains that produced β-1,3-glucanases, including Bacillus sp. 4 and Bacillus sp. 41 to attract fungus pathogen C. fructicola CL5 in chili inhibition ability. The results indicated Bacillus sp. 41. (secreted highest enzyme activity of 1,202± 0,04 U/mL after 12 hours of culture), higher than that of Bacillus sp. 4 (enzyme reached maximal of 0,85379 ± 0,04 U/mL after 15 hours of culture). The effect on C. fructicola CL5 growth prevention by the two Bacillus spp. in vitro models resulted in (Bacillus sp. 4: 50,73% after six days and Bacillus sp. 41: 57,39% after 12 days). In this result, β-1,3-glucanase was produced by Bacillus spp. positively contributed to the control of C. fructicola CL5 causing anthracnose disease in chili, which is equal to the commercial fungal pesticide Ridomil.

https://doi.org/10.26459/hueunijard.v133i3A.7379
PDF (Vietnamese)

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