IN VITRO SHOOT REGENERATION OF Adenosma indianum (Lour.) Merr. THROUGH CALLUS INDUCTION

Abstract

Adenosma indianum (Lour.) Merr. is a valuable medicinal plant distributed in coastal sandy areas but is no longer popular. This paper presents the results of in vitro shoot regeneration of Adenosma indiana (Lour.) Merr. through callus induction. The samples (leaf pieces, petioles and stems) were sterilized with HgCl₂ 0.1 % from 5 to 10 minutes. The suitable effective sterilization time is 10 min for leaves and stems and 6 min for petioles with the contamination rate of 18.52 %, 7.04 % and 0.00 %, respectively. The medium for callus formation from leaves was basal MS supplemented with 0.30 mg/l NAA, from petioles was 0.20 mg/l IBA and from stems was 0.50 mg/l NAA with the ratio of callus formation of 80.55 %, 66.67 % and 76.39 %, respectively. The basal MS medium supplemented with 0.50 mg/l BAP and 0.10 mg/l NAA was suitable for shoot generation from callus. The shoot forming ratio was 43.52 % with an average of 3.43 shoots/callus.