EFFECT OF NANOSILVER ON CALLUS INDUCTION AND SHOOT REGENERATION ABILITY FROM LEAF EXPLANTS OF STRAWBERRY (FRAGARIA X ANANASSA) CULTURED IN VITRO

Abstract

This study indicated the effect of nanosilver on inducing callus formation and shoot regeneration from ex vitro leaf explants via sterilization as well as its effect on the growth and development of strawberry’s shoots when added directly to the culture medium. The leaf explants sterilized with nanosilver of different concentrations and durations were compared with the control treated with HgCl₂, Ca(ClO)₂. All these samples were cultured in the MS medium supplemented with 1 mg/L TDZ, 0.1 mg/L IBA, 30 g/L sucrose và 8.5 g/L agar. The result showed that the treatment with the lowest infection rate (22.22 %) was treated with 0.5 g/L nanosilver for 20 minutes. The shoot regeneration rate (64.44 %), the number of shoots per explant (21), and the number of shoots longer than 1.5 cm (6.66) were the highest when treated with 0.2 g/L nanosilver for 20 minutes. The shoots obtained above were further cultured in the MS medium supplemented with 0.02 mg/L NAA. The height of plantlets (6.75 cm), the length of roots (5.13 cm) and the fresh weight of seedlings (0.71 g) were the highest in the treatment supplemented with 1 mg/L nanosilver; the number of roots (6.33) was the highest in the treatment with 2 mg/L nanosilver; the highest fresh dry mass (80.61 mg) and SPAD value (34.49) were the highest in the treatment with 1 mg/L nanosilver.