CLONING ESCHERICHIA COLI HARBORING EXPRESSION OF NEUTRALIZING EPITOPES (S1C AND S1C-CT24) OF PORCINE EPIDEMIC DIARRHEA VIRUS

Abstract

Porcine epidemic diarrhea virus (PEDV) belongs to family Coronaviridae and causes acute diarrhea in swine. The disease spreads rapidly and causes a high mortality rate. Thus, PEDV is considered as one of the major concerns of the pig industry around the world. The nucleotide sequence encoding epitope S₁C and peptide CT24 has been shown to induce neutralizing antibody responses, which are suitable for the development of recombinant PEDV vaccines. Recently, a novel strain of PEDV (HUA-PED45) belonging to the G2b group was discovered in Vietnam causing great losses to pig farms. Research of this PEDV strain at the molecular level in Vietnam is necessary to provide materials for the production of the recombinant PEDV vaccines or diagnostic kit. In this study, the S₁C alone or S₁C-CT24 fusion gene of the HUA-PED45 strain was synthesized, sequenced and cloned in expression vector pQE30 (Qiagen, Germany). These expression vectors transformed into E. coli strain M15 could provide the expression constructs for further experiments.