CLONING AND EXPRESSION OF GENE ENCODING PROTEIN p65 FROM MYCOPLASMA HYOPNEUMONIAE IN E. COLI BL21(DE3)
Vol. 127 No. 1C (2018), Special Issues - Conference
Vol. 127 No. 1C (2018)

CLONING AND EXPRESSION OF GENE ENCODING PROTEIN p65 FROM MYCOPLASMA HYOPNEUMONIAE IN E. COLI BL21(DE3)

Special Issues - Conference
https://doi.org/10.26459/hueuni-jns.v127i1C.4918
Published 2018-09-20
Huynh Van Chuong
Institute of Biotechnology, Hue University, Road No. 10, Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam
Dang Thanh Long
Institute of Biotechnology, Hue University, Road No. 10, Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam
Dinh Thi Bich Lan
University of Agriculture and Forestry, Hue University, 102 Phung Hung St., Hue, Vietnam
Hoang Thi Kim Hong
University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
Phung Thang Long
University of Agriculture and Forestry, Hue University, 102 Phung Hung St., Hue, Vietnam
Le Duc Thao
University of Agriculture and Forestry, Hue University, 102 Phung Hung St., Hue, Vietnam
Le Quoc Viet
Institute of Biotechnology, Hue University, Road No. 10, Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam
Vo Phuoc Khanh
Quang Nam University, 102 Hung Vuong, Quang Nam, Vietnam
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How to Cite

1.
Chương HV, Long Đặng T, Lân Đinh TB, Hồng HTK, Long PT, Thạo L Đức, Việt LQ, Khánh VP. CLONING AND EXPRESSION OF GENE ENCODING PROTEIN p65 FROM MYCOPLASMA HYOPNEUMONIAE IN E. COLI BL21(DE3). hueuni-jns [Internet]. 2018Sep.20 [cited 2024Nov.15];127(1C):11-9. Available from: http://222.255.146.83/index.php/hujos-ns/article/view/4918
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Abstract

In this study, we successfullycloned and expressed the p65 gene encoding for p65 proteinof Mycoplasma hyopneumonia (M. hyopneumonia) isolated from pig lungs collected in Thua Thien Hue province, Vietnam. The p65 gene was amplified and cloned into pET200/D-TOPO vector and then transformed into the E. coli BL21 (DE3) strain. The results showed that the p65 gene segment was 936 bp, identical to the published p65 gene on GenBank (accession number: CP003131.1), encoding a polypeptide chain of 311 amino acid residues, identical to anamino acid sequence of a protein on GenBank (accession number: AAB67173.1). The denatured SDS-PAGE analysis showed a protein band of 37 kDa which corresponded to 6×His-p65 fusion protein.

https://doi.org/10.26459/hueuni-jns.v127i1C.4918
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