CLONING AND EXPRESSION OF GENE ENCODING ANTIGEN GLYCOPROTEIN C OF DUCK PLAGUE VIRUS ISOLATED FROM THUA THIEN HUE PROVINCE

Abstract

In this study, we cloned and expressed the UL44 gene encoding for glycoprotein C (gC) of duck virus enteritis. The length of the UL44 gene segment extracted from total DNA of duck livers was 1296 bp, identical to a gene sequence in the Genbank (accession number: EU076811.1). The polypeptide chain encoded of 431 amino acid residues was identical to a polypeptide chain in the Genbank (accession number: ABW82653.1). The SDS electrophoresis results showed that fusion protein 6xHis-gC had a molecular weight of approximately 50 kDa.