Isolation and evaluation anti-pathogenic activities of Lactococcus garvieae from shrimp intestinal system
Vol. 128 No. 1E (2019), Special Issues - Conference
Vol. 128 No. 1E (2019)

Isolation and evaluation anti-pathogenic activities of Lactococcus garvieae from shrimp intestinal system

Special Issues - Conference
https://doi.org/10.26459/hueuni-jns.v128i1E.5427
Published 2019-10-25
Lê Mỹ Tiểu Ngọc
Viện Công nghệ sinh học, Đại học Huế
Đặng Quang Nguyên
Trường đại học Khoa học, Đại học Huế, 77 Nguyễn Huệ, Huế, Thừa Thiên Huế, Việt Nam
Đỗ TrầnHương Duyên
Viện Công nghệ sinh học, Đại học Huế, tỉnh lộ 10, Phú Thượng, Phú Vang, Thừa Thiên Huế, Việt Nam
Trần Thúy Lan
Viện Công nghệ sinh học, Đại học Huế, tỉnh lộ 10, Phú Thượng, Phú Vang, Thừa Thiên Huế, Việt Nam
Nguyễn Quang Đức Tiến
Trường đại học Khoa học, Đại học Huế, 77 Nguyễn Huệ, Huế, Thừa Thiên Huế, Việt Nam
Nguyễn Duy Quỳnh Trâm
Trường đại học Nông Lâm, Đại học Huế, 102 Phùng Hưng, Huế, Thừa Thiên Huế, Việt Nam
Nguyễn Đức Huy
Viện Công nghệ sinh học, Đại học Huế, tỉnh lộ 10, Phú Thượng, Phú Vang, Thừa Thiên Huế, Việt Nam
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1.
Ngọc LMT, Nguyên Đặng Q, Duyên Đỗ T, Lan TT, Tiến NQ Đức, Trâm NDQ, Huy N Đức. Isolation and evaluation anti-pathogenic activities of Lactococcus garvieae from shrimp intestinal system. hueuni-jns [Internet]. 2019Oct.25 [cited 2024Nov.14];128(1E):77-86. Available from: http://222.255.146.83/index.php/hujos-ns/article/view/5427
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Abstract

The bioproducts to inhibit pathogenic bacteria have become an alternative to replace antibiotics. In this study, we isolated 17 potential lactic bacteria strains from 23 shrimp samples in Thua Thien Hue province with Vibrio parahaemolyticus inhibition activity. The molecular identification based on 16S rRNA nucleotide sequence comparison indicated that 4 strains are highly identity with Lactococcus garvieae. The isolate with the highest antagonistic activity was selected to evaluate the growth inhibition against Vibrio spp., Escherichia coli ATCC 85922 and Staphylococcus aureus ATCC 25023. The inhibition zone demonstrated that the isolate was capable to inhibit various Vibrio spp. with the highest inhibition diameter of 23 mm. Meanwhile, the isolate showed a 15 mm and 11 mm inhibition diameter against S. aureus ATCC 25023 and E. coli ATCC 85922, respectively. This is the first study in Vietnam conducting the isolation and evaluation of L. garvieae against pathogenic bacteria causing disease on shrimps and other aqua animals.

https://doi.org/10.26459/hueuni-jns.v128i1E.5427
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References

  1. Abdullah SA, Osman MM. Isolation and identification of lactic acid bacteria from raw cow milk, white cheese and rob in Sudan. Pak J Nutr. 2010;9(12):1203-6.
  2. Kaktcham PM, Zambou NF, Tchouanguep FM, El-Soda M, Choudhary MI. Antimicrobial and safety properties of Lactobacilli isolated from two cameroonian traditional fermented foods. Sci Pharm. 2012 Jan-Mar;80(1):189-203.
  3. Brashears MM, Jaroni D, Trimble J. Isolation, selection, and characterization of lactic acid bacteria for a competitive exclusion product to reduce shedding of Escherichia coli O157:H7 in cattle. J Food Prot. 2003 Mar;66(3):355-63.
  4. Chen YJ, Son KS, Min BJ, Cho JH, Kwon OS, Kim IH. Effects of dietary probiotic on growth performance, nutrients digestibility, blood characteristics and fecal noxious gas content in growing pigs. Asian-Australas J Anim Sci. 2005;18(10):1464-8.
  5. Villani F, Aponte M, Blaiotta G, Mauriello G, Pepe O, Moschetti G. Detection and characterization of a bacteriocin, garviecin L1-5, produced by Lactococcus garvieae isolated from raw cow's milk. J Appl Microbiol. 2001 Mar;90(3):430-9.
  6. Holzapfel WH, Geisen R, Schillinger U. Biological preservation of foods with reference to protective cultures, bacteriocins and food-grade enzymes. Int J Food Microbiol. 1995 Jan;24(3):343-62.
  7. Nettles CG, Barefoot SF. Biochemical and genetic characteristics of bacteriocins of food-associated lactic acid bacteria. J Food Prot. 1993 Apr;56(4):338-56.
  8. Wang CY, Shie HS, Chen SC, Huang JP, Hsieh IC, Wen MS, et al. Lactococcus garvieae infections in humans: possible association with aquaculture outbreaks. Int J Clin Pract. 2007 Jan;61(1):68-73.
  9. Borrero J, Brede DA, Skaugen M, Diep DB, Herranz C, Nes IF, et al. Characterization of garvicin ML, a novel circular bacteriocin produced by Lactococcus garvieae DCC43, isolated from mallard ducks (Anas platyrhynchos). Appl Environ Microbiol. 2011 Jan;77(1):369-73.
  10. Tosukhowong A, Zendo T, Visessanguan W, Roytrakul S, Pumpuang L, Jaresitthikunchai J, et al. Garvieacin Q, a novel class II bacteriocin from Lactococcus garvieae BCC 43578. Appl Environ Microbiol. 2012 Mar;78(5):1619-23.
  11. Maldonado-Barragan A, Cardenas N, Martinez B, Ruiz-Barba JL, Fernandez-Garayzabal JF, Rodriguez JM, et al. Garvicin A, a novel class IId bacteriocin from Lactococcus garvieae that inhibits septum formation in L. garvieae strains. Appl Environ Microbiol. 2013 Jul;79(14):4336-46.
  12. Ovchinnikov KV, Chi H, Mehmeti I, Holo H, Nes IF, Diep DB. Novel group of leaderless multipeptide bacteriocins from gram-positive bacteria. Appl Environ Microbiol. 2016 Sep 1;82(17):5216-24.
  13. Suneel D, Basappa K. Identification and characterization of Lactococcus garvieae and antimicrobial activity of its bacteriocin isolated from cow’s milk. Asian J Pharm Clin Res. 2013;6(3):104-8.
  14. Kandler O, Weiss N. Regular, non-sporing gram-positive rods. In: Sneath HA, Mair NS, Sharpe ME, Holt JG, editors. Bergey’s Manual of Systematic Bacteriology. Baltimore: Williams & Wilkins; 1986.
  15. Tagg JR, McGiven AR. Assay system for bacteriocins. Appl Microbiol. 1971 May;21(5):943.
  16. Iyapparaj P, Maruthiah T, Ramasubburayan R, Prakash S, Kumar C, Immanuel G, et al. Optimization of bacteriocin production by Lactobacillus sp. MSU3IR against shrimp bacterial pathogens. Aquat Biosyst. 2013 Jun 1;9(1):12.
  17. Sambrook S., Russell RD. Molecular Cloning: A Laboratory Manual. 3rd ed. New York: Cold Spring Harbor; 2003.
  18. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol. 2018 Jun 1;35(6):1547-9.
  19. Forhada MH, Rahmana SMK, Rahmana S, Saikota FK, Biswasb KC. Probiotic properties analysis of isolated lactic acid bacteria from buffalo milk. Arch Clin Microbiol. 2015;7(1):5.
  20. Manzoor A, Ul-Haq I, Baig S, Qazi JI, Seratlic S. Efficacy of locally isolated lactic acid bacteria against antibiotic-resistant uropathogens. Jundishapur J Microbiol. 2016 Jan;9(1):e18952.
  21. Meyrand A, Boutrand-Loei S, Ray-Gueniot S, Mazuy C, Gaspard CE, Jaubert G, et al. Growth and enterotoxin production of Staphylococcus aureus during the manufacture and ripening of Camembert-type cheeses from raw goats' milk. J Appl Microbiol. 1998 Sep;85(3):537-44.
  22. Morin M, Lariviere S, Lallier R. Pathological and microbiological observations made on spontaneous cases of acute neonatal calf diarrhea. Can J Comp Med. 1976 Jul;40(3):228-40.
  23. Abdelfatah EN, Mahboub HHH. Studies on the effect of Lactococcus garvieae of dairy origin on both cheese and Nile tilapia (O. niloticus). Int J Vet Sci Med. 2018 Dec;6(2):201-7.
  24. Alomar J. Study of physiological properties of Lactococcus lactis and Lactococcus garvieae for the control of Staphylococcus aureus in technology Cheese. Nancy: National Polytechnic Institute of Lorraine; 2007.
  25. Delbes-Paus C, Dorchies G, Chaabna Z, Callon C, Montel MC. Contribution of hydrogen peroxide to the inhibition of Staphylococcus aureus by Lactococcus garvieae in interaction with raw milk microbial community. Food Microbiol. 2010 Oct;27(7):924-32.
  26. Shehata MG, El Sohaimy SA, El-Sahn MA, Youssef MM. Screening of isolated potential probiotic lactic acid bacteria for cholesterol lowering property and bile salt hydrolase activity. Ann Agric Sci. 2016;61(1):65-75.
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