Effects of medium composition on Xa den (Ehretia asperula Zoll. et Mor.) cell cultures
Vol. 129 No. 1A (2020), Hue University Journal of Science: Natural Science
Vol. 129 No. 1A (2020)

Effects of medium composition on Xa den (Ehretia asperula Zoll. et Mor.) cell cultures

Hue University Journal of Science: Natural Science
https://doi.org/10.26459/hueunijns.v129i1A.5676
Published 2020-03-20
Pham Thi My Tram
Thu Dau Mot University, 6 Tran Van On St., Thu Dau Mot, Binh Duong, Vietnam
Ngo Ke Suong
Institute of Tropical Biology, Ho Chi Minh City, 9/621 Hanoi Road., Dist. Thu Duc, Ho Chi Minh City, Vietnam
Le Thi Thuy Tien
VNUHCM – University of Technology, 268 Ly Thuong Kiet St., Dist. 10, Ho Chi Minh City, Vietnam
PDF (Vietnamese)

Keywords

callus
cell suspension cultures
Ehretia asperula Zoll. et Mor.
xa den Ehretia asperula Zoll. et Mor.
huyền phù tế bào
mô sẹo
xạ đen

How to Cite

1.
Mỹ Trâm PT, Sương NK, Thủy Tiên LT. Effects of medium composition on Xa den (Ehretia asperula Zoll. et Mor.) cell cultures. hueuni-jns [Internet]. 2020Mar.20 [cited 2024Nov.14];129(1A):31-4. Available from: http://222.255.146.83/index.php/hujos-ns/article/view/5676
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Abstract

Xa den (Ehretia asperula Zoll. et Mor.) belongs to the Boraginaceae family and appears in Southeast Asian countries. Xa den is often used to treat diseases such as tumors, emphysema, and detoxification. The effects of plant growth regulators and carbon sources on the formation and proliferation of callus from Xa den leaves in vitro were studied. The results show that callus proliferates well on the medium supplemented with 30 mg/L glucose, 0.4 mg/L 2,4-D, and 0.1 mg/L BA. In this medium, the callus is yellowish-green, friable, and rapid proliferating. After the first subculture, the callus is milky white and friable after 10 weeks of culture. This callus is used as a material for cell suspension cultures. Suspension cells grow in the dark better than in the light with the highest biomass after four weeks of culture (149.933 g/L).

https://doi.org/10.26459/hueunijns.v129i1A.5676
PDF (Vietnamese)

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