Improved expression of catechol 1,2-dioxygenase gene from Burkholderia cepacia in Escherichia coli
Vol. 129 No. 1D (2020), Original Article
Vol. 129 No. 1D (2020)

Improved expression of catechol 1,2-dioxygenase gene from Burkholderia cepacia in Escherichia coli

Original Article
https://doi.org/10.26459/hueunijns.v129i1D.5974
Published 2020-11-24
Dang Van Thanh
Department of Biotechnology, University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
Hoang Anh Thi
Department of Biotechnology, University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
Tran Gia Cat Tuong
Department of Biotechnology, University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
Pham Thi Huyen Trang
Department of Biotechnology, University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
Le Thi Ha Thanh
Department of Biotechnology, University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
Nguyen Hoang Loc
Department of Biotechnology, University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
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Keywords

Escherichia coli M15
CAT1
gene expression
dibenzofuran

How to Cite

1.
Dang VT, Hoang AT, Tran GCT, Pham THT, Ha Thanh LT, Nguyen HL. Improved expression of catechol 1,2-dioxygenase gene from Burkholderia cepacia in Escherichia coli . hueuni-jns [Internet]. 2020Nov.24 [cited 2024Nov.23];129(1D):61-5. Available from: http://222.255.146.83/index.php/hujos-ns/article/view/5974
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Abstract

Catechol 1,2-dioxygenase (CAT1) is a key enzyme for the ortho-cleavage pathway involved in the degradation of dibenzofuran, a dioxin derivative, which is a highly toxic environmental pollutant. The present study aims to investigate appropriate culture conditions for enhancing the expression of the cat1 gene encoding CAT1 enzyme from Burkholderia cepacia DF4 in Escherichia coli M15. The optimized culture conditions for gene expression are cell density at the time of induction, shaking speed, induction temperature, induction time, and inducer concentration. The highest level for CAT1 was obtained at the IPTG concentration of 1.2 mM, 10 hours after induction at 35 °C, shaking speed 200 rpm with cell density at OD600 0.7.

https://doi.org/10.26459/hueunijns.v129i1D.5974
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