In vitro propagation of Dendrobium anosmum mutation
Vol. 131 No. 1A (2022), Original Article
Vol. 131 No. 1A (2022)

In vitro propagation of Dendrobium anosmum mutation

Original Article
https://doi.org/10.26459/hueunijns.v131i1A.6556
Published 2022-03-31
Le Vinh Thien
Institute of Biotechnology, Hue University, Phu Thuong, Phu Vang, Thua Thien Hue
Truong Thi Ly Na
Quang Tri Technical College, 179 Ly Thuong Kiet st., Quang Tri, Vietnam
Nguyen Thi Bich Thu
Quang Tri Technical College, 179 Ly Thuong Kiet St., Quang Tri, Vietnam
Nguyen Thi Diem
Institute of Biotechnology, Hue University, Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam
Nguyen Thi Oanh
Institute of Biotechnology, Hue University, Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam
Nguyen Thi Kim Cuc*
Institute of Biotechnology, Hue University, Phu Thuong, Phu Vang, Thua Thien Hue
PDF (Vietnamese)

Keywords

Giả hạc đột biến
gieo hạt
in vitro Dendrobium anosmum
in vitro
mutant orchids
seedings

How to Cite

1.
Lê VT, Trương TLN, Nguyễn TBT, Nguyen TD, Nguyễn TO, Nguyễn TKC. In vitro propagation of Dendrobium anosmum mutation. hueuni-jns [Internet]. 2022Mar.31 [cited 2024Nov.14];131(1A):5-15. Available from: http://222.255.146.83/index.php/hujos-ns/article/view/6556
ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver

Download Citation

Endnote/Zotero/Mendeley (RIS) BibTeX
Crossref
Scopus
Google Scholar
Europe PMC

Abstract

Currently, mutant orchids are very interested in orchid lovers who want to collect, but the price of mutant orchids is costly leading to many orchid lovers are unable to own it. This study aims to create mutant orchid plantlets with high quantity and low cost via in vitro propagation method. From the self-pollinated six months old seedpods, we have successfully propagated the orchid mutant seedlings. The results showed that the suitable medium for mutant orchid seed germination and creating protocorm was the basic MS medium supplemented with 0.5 mg/L BA with a rate of up to 93%. The medium for shoot formation and shoot development from protocorm was the best on MS medium added with 1.0 mg/L BA and 0.5 mg/L IBA with a rate of 88%. The rooting induction was 99.06% and the root length reached 5.34 cm with an average of 8.53 roots/shoot when shoots cultured on MS medium supplemented with 1.0 mg/L NAA. The survival rate of in vitro Dendrobium anosmum mutant seedlings after 60 days in the nursery stage was 84.9% on coir and rice husks substrates (1:1).

https://doi.org/10.26459/hueunijns.v131i1A.6556
PDF (Vietnamese)

References

  1. Da Silva JAT, Zeng S, Cardoso JC, Dobránszki J, Kerbauy GB. In vitro flowering of Dendrobium. Plant Cell, Tissue Organ Cult. 2014;119(3):447-56.
  2. Da Silva JAT, Cardoso JC, Dobránszki J, Zeng S. Dendrobium micropropagation: a review. Plant Cell Rep. 2015;34(5):671-704.
  3. Rattana K, Sangchanjiradet S. Micropropagation of Dendrobium signatum Rchb.f.. Pertanika Journal of Tropical Agricultural science. 2017;40(4).
  4. Maharjan S, Thakuri L Sen, Thapa BB, Pradhan S, Pant KK, Joshi GP, et al. In vitro propagation of the endangered orchid Dendrobium chryseum Rolfe from protocorms culture. Nepal Journal of Science and Technology. 2020;19(1):39-47.
  5. Thanh NV. The danger of depleting forest orchids [Internet]. Agricultural Vietnam Artical. 2015 [cited 2021 May 10]. Available from: https://nongnghiep.vn/nguy-co-can-kiet-lan-rung-d137678.html
  6. Martin KP, Madassery J. Rapid in vitro propagation of Dendrobium hybrids through direct shoot formation from foliar explants, and protocorm-like bodies. Sci Hortic (Amsterdam). 2006;108(1):95-9.
  7. Pant B, Thapa D. In vitro mass propagation of an epiphytic orchid, Dendrobium primulinum Lindl. through shoot tip culture. African J Biotechnol. 2012;11(42):9970-4.
  8. Puchooa D. Comparison of different culture media for the in vitro culture of Dendrobium (Orchidaceae). Int J Agric Biol. 2004;6:884-8.
  9. Lan VN, Anh NTL. Nhân giống in vitro loài lan bản địa Dendrobium nobile Lindl. Tạp chí Khoa học và Phát triển. 2013;11(7):917-25.
  10. Maharjan S, Pradhan S, Thapa BB, Pant B. In vitro propagation of endangered orchid, Vanda pumila Hook. f. through protocorms culture. Am J Plant Sci. 2019;10(07):1220.
  11. Thắm ĐT, H’Yon NB, Hằng NTT, Khiêm ĐV, Duy NV, Vinh TT, Lợi QV, Công VK. Vi nhân giống lan Nhất điểm hoàng (Denrobium heterocarpum Lindl). Tạp Chí Công Nghệ Sinh Học. 2018;16(1):127-35.
  12. Murashige T, Skoog E. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plantarurn. 1962;(15):473-97.
  13. Hiền PTT, Đính NV. Nhân giống lan Đai châu đỏ (Rhynchostylisgigantea L.) bằng công nghệ nuôi cấy in vitro. VNU J Sci Nat Sci Technol. 2017;33(1):48-57.
  14. Chiến HĐ, Đính NV, Trang VT, Xuân ĐT, Bằng CP. Nhân giống in vitro lan Mokara thông qua protocorm-like body từ mô lá. TNU J Sci Technol. 2020;225(08):280-5.
  15. Duyên NTM. Quy trình vi nhân giống lan Giả hạc (Dendrobium anosmum). AGU Int J Sci. 2021;27(1):73-82.
  16. Diễm NT, Oanh NT, Tâm HT, Thọ NH, Cúc NTK. Cultivation of Dendrobium anosmum Di Linh from in vitro seedlings. Hue Univ J Sci Nat Sci. 2021;130(1A):107-15.
  17. De LC, Rao AN, Rajeeva PK, Srivastava M. Morphological characterization in Dendrobium species. J Biosci. 2015;4(1):1198-215.
  18. Liên ĐQ, Hương ĐTT, Việt NV, Hưng VT. Ứng dụng kỹ thuật nuôi cấy in vitro trong nhân giống lan phi điệp tím Hoà Bình (Dendrobium anosmum Lindley). Tạp chí Khoa học và Công nghệ Lâm nghiệp. 2019;6:9-16.
  19. Trang NQ, Huệ VT, Ninh KTH, Thơ NT. Nhân giống lan phi điệp tím (Dendrobium anosmum). Tạp chí Khoa Học và Công Nghệ Lâm Nghiệp. 2013;3(1):16-21.
  20. Riva SS, Islam A, Hoque ME. In vitro regeneration and rapid multiplication of Dendrobium bensoniae, an indigenous ornamental orchid. Agric. 2016;14(2):24-31.
  21. Murti RH, Yeoung YR. Effects of BA and IBA concentrations and subculture frequent on meristem culture of strawberry. ARPN J Agric Biol Sci. 2013;8(5):405-10.
  22. Kang H, Kang KW, Kim DH, Sivanesan I. In vitro propagation of Gastrochilus matsuran (Makino) Schltr., an endangered epiphytic orchid. Plants. 2020;9(4):524.
  23. Utami ESW, Hariyanto S, Manuhara YSW. In vitro propagation of the endangered medicinal orchid, Dendrobium lasianthera J.J.Sm through mature seed culture. Asian Pac J Trop Biomed [Internet]. 2017;7(5):406-10.
  24. Kumar KV, Fatmi U. Effects of IBA and NAA on shoot growth of cuttings of various ornamental plants in water as rooting medium. J Pharmacogn Phytochem. 2021;10(2):685-7.
Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Copyright (c) 2021 Array