Effect of cyanobacterial extract on in vitro propagation of Dendrobium anosmum Lindl.
Vol. 131 No. 1C (2022), Original Article
Vol. 131 No. 1C (2022)

Effect of cyanobacterial extract on in vitro propagation of Dendrobium anosmum Lindl.

Original Article
https://doi.org/10.26459/hueunijns.v131i1C.6564
Published 2022-09-30
Truong Thi Bich Phuong
University of Sciences, Hue University, 77 Nguyen Hue, Hue, Vietnam
Tong Van Bao Thanh
University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
Phan Thi Thao Nguyen
University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
Nguyen Duc Tuan
University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
Lam Thi Ngoc Thuy
University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
Hoang Thi Kim Hong
University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
Nguyen Thi Thu Lien*
University of Sciences, Hue University, 77 Nguyen Hue, Hue, Vietnam
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Keywords

Dendrobium anosmum
dịch chiết vi khuẩn lam
nhân giống in vitro
nhân nhanh chồi
tạo rễ cyanobacterial extract
in vitro propagation
shoot multiplication
rooting

How to Cite

1.
Trương TBP, Tống VBT, Phan TTN, Nguyễn Đức T, Lâm TNT, Hoàng TKH, Nguyễn TTL. Effect of cyanobacterial extract on in vitro propagation of Dendrobium anosmum Lindl. hueuni-jns [Internet]. 2022Sep.30 [cited 2024Nov.14];131(1C):73-82. Available from: http://222.255.146.83/index.php/hujos-ns/article/view/6564
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Abstract

Protocorms of 4-week-old Dendrobium anosmum Lindl. were used as initial material for in vitro propagation. The stock extract of Arthrospira sp. was prepared by grinding 1 g of fresh biomass in 100 mL of distilled water. The in vitro propagation results show that the cyanobacterial extract has the effect of enhancing shoot multiplication and rooting of Dendrobium anosmum. An MS basal medium supplemented with 6 g·L–1 agar, 30 g·L–1 saccharose, and 1.5 mg·L–1 BAP in combination with 20 mL·L–1 cyanobacteria extract was suitable for protocorm multiplication with a protocorm cluster diameters of 2.43 cm. An MS medium supplemented with 6 g·L–1 agar, 30 g·L–1 sucrose, and 1.0 mg·L–1 BAP combined with 20 mL·L–1 cyanobacterial extract was optimal for shoot multiplication. The number of shoots/explant reached 4.7 and a shoot height of 1.37 cm. An MS medium supplemented with 6 g·L–1 agar, 30 g·L–1 sucrose, and 1.5 mg·L–1 NAA in combination with 30 mL·L–1 cyanobacteria extract was the most suitable for rooting with 4.87 roots/bud; the root length was 0.74 cm, and the shoot height was 2.76 cm. These results would open up the application of cyanobacterial extract to reduce costs in plant tissue culture technology.

https://doi.org/10.26459/hueunijns.v131i1C.6564
PDF (Vietnamese)

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