Detection of hypoglycemic drugs adulterated in herbal products with high-performance liquid chromatography
Vol. 132 No. 1A (2023), Original Article
Vol. 132 No. 1A (2023)

Detection of hypoglycemic drugs adulterated in herbal products with high-performance liquid chromatography

Original Article
https://doi.org/10.26459/hueunijns.v132i1A.6975
Published 2023-03-31
Le Thi Bao Tram
University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen St., Hue, Vietnam
Ta Nguyen Khanh Ha
University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen St., Hue, Vietnam
Hoang Thi Minh Nguyet
Hue Central Hospital, 16 Le Loi St., Hue, Vietnam
Pham Thi Thanh Ha
Hanoi University of Pharmacy, 13–15 Le Thanh Ton St., Hanoi, Vietnam
Nguyen Thi Kieu Anh
Hanoi University of Pharmacy, 13–15 Le Thanh Ton St., Hanoi, Vietnam
Nguyen Hai Phong
University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
Huynh Van Chung
Buon Ma Thuot Medical University, 298 Ha Huy Tap St., Buon Ma Thuot, Daklak, Vietnam
Dao Thi Cam Minh*
University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen St., Hue, Vietnam
PDF (Vietnamese)

Keywords

hypoglycemic drug
herbal product
high-performance liquid chromatography thuốc hạ glucose máu
chế phẩm dược liệu
sắc ký lỏng hiệu năng cao

How to Cite

1.
Lê TBT, Tạ NKH, Hoàng TMN, Phạm TTH, Nguyễn TKA, Nguyễn HP, Huỳnh VC, Đào TCM. Detection of hypoglycemic drugs adulterated in herbal products with high-performance liquid chromatography. hueuni-jns [Internet]. 2023Mar.31 [cited 2024Nov.23];132(1A):65-73. Available from: http://222.255.146.83/index.php/hujos-ns/article/view/6975
ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver

Download Citation

Endnote/Zotero/Mendeley (RIS) BibTeX
Crossref
Scopus
Google Scholar
Europe PMC

Abstract

Circular No. 10/2021/TT-BYT stipulated that several hypoglycemic drugs, such as metformin hydrochloride, are prohibited from adulterating medicinal herbal preparations. Therefore, this study aims to develop a procedure to detect five hypoglycemic drugs, including metformin hydrochloride, glibenclamide, gliclazide, glimepiride, and glipizide, adulterated in herbal preparations by using the high-performance liquid chromatography (HPLC) method. The chromatographic program includes the methanol:phosphate buffer pH 3 mobile phase 70/30 (v/v), chromatographic column C18 (250 × 4.6 mm, 5 μm), and analysis time of 25 minutes. The procedure has high specificity, accuracy and precision, according to AOAC. We detected six herbal products reacting positively with glibenclamide and one with both metformin hydrochloride and glibenclamide.

https://doi.org/10.26459/hueunijns.v132i1A.6975
PDF (Vietnamese)

References

  1. Bộ Y tế. Thông tư 10/2021/TT-BYT Quy định Danh mục chất cấm sử dụng trong sản xuất, kinh doanh thực phẩm chức năng bảo vệ sức khỏe. Hà Nội: Bộ Y tế; 2021.
  2. Li N, Cui M, Lu X, Qin F, Jiang K, Li F. A rapid and reliable UPLC-MS/MS method for the identification and quantification of fourteen synthetic anti-diabetic drugs in adulterated Chinese proprietary medicines and dietary supplements, Biomed Chromatogr. 2010;24(11):1255-61.
  3. Thắng VN, Hùng TM, Dung VTP, Chí VD, Xây dựng phương pháp HPLC để phân tích đồng thời một số thuốc hóa dược điều trị đái tháo đường có thể trộn trong một số chế phẩm từ dược liệu. Tạp chí Kiểm nghiệm thuốc. 2017;15(55):16-21.
  4. Kumasaka K, Kojima T, Honda H, Doi K. Screening and Quantitative Analysis for Sulfonylurea-Type Oral Antidiabetic Agents in Adulterated Health Food Using Thin-Layer Chromatography and High-Performance Liquid Chromatography, Journal of Health Science. 2005);51(4):453-60.
  5. Minh ĐTC, Hà PTT, Anh NTK. Nghiên cứu phát hiện các thuốc giảm đau, chống viêm, giảm glucose máu trộn trái phép trong chế phẩm Đông dược bằng sắc ký lớp mỏng hiệu năng cao. Tạp chí Dược học. 2019;59(516):57-62.
  6. Bogusz MJ, Hassan H, Al-Enazi E, Ibrahim Z, Al-Tufail M, Application of LC–ESI–MS–MS for detection of synthetic adulterants in herbal remedies, Journal of Pharmaceutical and Biomedical Analysis, 2006;41:554–64.
  7. Cui M, Li N, Qin F, Li F, Xiong Z. Simultaneous Determination of 14 Illegal Adulterants in Chinese Proprietary Medicines Using Reversed-Phase Ion-Pair LC. Chroma. 2010;72:1189-94.
  8. AOAC Official Methods of Analysis. Appendix F: Guidelines for Standard Method Performance Requirements; 2016.
  9. Pang W, Yang H, Wu Z, Huang M, Hu J. LC-MS–MS in MRM Mode for Detection and Structural Identification of Synthetic Hypoglycemic Drugs Added Illegally to ‘Natural’ Anti-Diabetic Herbal Products. Chroma. 2009;70:1353-9.
  10. Bộ Y tế. Dược thư quốc gia Việt Nam. Hà Nôi: Nxb Y học; 2018.
Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Copyright (c) 2023 Array