Polysaccharide extraction from Myxopyrum smilacifolium trunk and its antioxidant capacity
Vol. 132 No. 1D (2023), Original Article
Vol. 132 No. 1D (2023)

Polysaccharide extraction from Myxopyrum smilacifolium trunk and its antioxidant capacity

Original Article
https://doi.org/10.26459/hueunijns.v132i1D.7012
Published 2023-12-30
Le Trung Hieu*
Faculty of Chemistry, Hue University of Sciences, Hue University, Hue, Vietnam
Le Lam Son
letrunghieu.chem@gmail.com
Nguyen Dang Giang Chau
letrunghieu.chem@gmail.com
Nguyen Minh Nhung
Center for Measurement, Testing and Scientific information, Department of Science and Technology Thua Thien Hue province, Hue, Vietnam
Tran Thanh Minh
Faculty of Chemistry, Hue University of Sciences, Hue University, Hue, Vietnam
Ho Xuan Anh Vu
Faculty of Chemistry, Hue University of Sciences, Hue University, Hue, Vietnam
Le Thuy Trang
Faculty of Chemistry, Hue University of Sciences, Hue University, Hue, Vietnam
Nguyen Viet Thang
Faculty of Biology, Hue University of Sciences, Hue University, Hue, Vietnam
Nguyen Quang Man
University of Medicine and Pharmacy, Hue University, Hue, Vietnam
Tran Thi Van Thi
Faculty of Chemistry, Hue University of Sciences, Hue University, Hue, Vietnam
PDF

Keywords

Myxopyrum smilacifolium
polysaccharides extraction
antioxidant activities

How to Cite

1.
Hieu LT, Le Lam S, Nguyen Dang Giang C, Nguyen Minh N, Tran Thanh M, Ho Xuan Anh V, Le Thuy T, Nguyen Viet T, Nguyen Quang M, Tran Thi Van T. Polysaccharide extraction from Myxopyrum smilacifolium trunk and its antioxidant capacity. hueuni-jns [Internet]. 2023Dec.30 [cited 2024Nov.23];132(1D):63-71. Available from: http://222.255.146.83/index.php/hujos-ns/article/view/7012
ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver

Download Citation

Endnote/Zotero/Mendeley (RIS) BibTeX
Crossref
Scopus
Google Scholar
Europe PMC

Abstract

As a traditional medicinal plant in Vietnam, Myxopyrum smilacifolium has been used for a long history to treat cough, nervous disorders, numbness, rheumatism, and cephalalgia. Nevertheless, reports on the antioxidant activity and extraction of M. smilacifolium polysaccharides are still very rare. This study was designed to extract a high yield of polysaccharides from the M. smilacifolium trunk and characterize it. As a result, the maximum yield of the polysaccharides of 5.13 ± 0.05% was obtained with an extraction time of three hours, extraction temperature of 100 oC, the ratio of water to sample of 1:50 as extraction solution, extraction number of times 3, and the ratio of ethanol to extract volume 5:1 (v/v). Polysaccharides was characterized by Fourier transform infrared spectroscopy (FT-IR) and high-performance gel-permeation chromatography (HPGPC). The average molecular weight of extracted polysaccharides was around 3.78 × 105 Da. In vitro assays dan impressive antioxidant activities of the extracted polysaccharides, containing 0.2423 ± 0.0028 mg GA/g or 0.2142 ± 0.0007 μmol AS/g. The IC50 values of polysaccharides in the DPPH and ABTS methods were 0.89 mg/mL and 3.85 mg/mL, respectively.  These findings exhibited the potential for application or further research and development of polysaccharides from Myxopyrum smilacifolium.

https://doi.org/10.26459/hueunijns.v132i1D.7012
PDF

References

  1. Barclay TG, Day CM, Petrovsky N, Garg S. Review of polysaccharide particle-based functional drug delivery. Carbohydrate Polymers. 2019;221:94-112.
  2. Chaiklahan R, Chirasuwan N, Triratana P, Loha V, Tia S, Bunnag B. Polysaccharide extraction from Spirulina sp. and its antioxidant capacity. International Journal of Biological Macromolecules. 2013;58:73-78.
  3. Tzianabos AO. Polysaccharide immunomodulators as therapeutic agents: structural aspects and biologic function. Clinical Microbiology Reviews 2000;13(4):523-533.
  4. Liu Y, Sun Y, Huang G. Preparation and antioxidant activities of important traditional plant polysaccharides. International Journal of Biological Macromolecules 2018;111:780-786.
  5. Chen L, Huang G. The antiviral activity of polysaccharides and their derivatives. International Journal of Biological Macromolecules. 2018;115:77-82.
  6. Khatua S, Acharya K. Alkaline extractive crude polysaccharide from Russula senecis possesses antioxidant potential and stimulates innate immunity response. Journal of Pharmacy and Pharmacology. 2017;69(12):1817-1828.
  7. Hambali E, Wibawa Sakti SC, Fahmi MZ, Wahyudianto FE, Nuryono, Yessi P, et al. In Effect of Extraction Time and Na2CO3 Concentration on The Characteristics of Alginate Extracted from Sargassum sp. IOP Conference Series: Earth and Environmental Science. 2018;209(1):012033.
  8. Tang W, Liu D, Yin JY, Nie SP. Consecutive and progressive purification of food-derived natural polysaccharide: Based on material, extraction process and crude polysaccharide. Trends in Food Science & Technology. 2020;99:76-87.
  9. Praveen R, Nair AS. Preliminary phytochemical screening of root extracts of Myxopyrum smilacifolium Blume. Asian Journal of Biomedical and Pharmaceutical Sciences. 2014; 4(38):41-45.
  10. Siju EN, Samu J, Minil M, Rajalakshmi GR. Antioxidant activity of myxopyrum smilacifolium blume. Asian Journal of Pharmaceutical and Clinical Research. 2015;8:119-21
  11. Vuong NQ, Chien VV, Hue NT, Hang PT, Hieu TV, Nhiem NX, et al. Chemical constituents from the roots of Myxopyrum smilacifolium. Vietnam Journal of Science and Technology. 2021;59(4):498-506.
  12. Peeriga R, Bonnth CK. Pharmacognostical Investigation and Preliminary Phytochemical Screening of Leaves of Myxopyrum Smilacifolium B. Pharmacognosy Journal. 2016;8(2).
  13. Le TH, Thi TTV, Tran VK, Vu HXA, Tran TM, Chau NDG, et al. Structural Characterization of Mannoglucan Isolated from Ophiocordyceps sobolifera and Its Antioxidant Activities. ACS omega. 2022;7(11):9397-9405.
  14. Dubois M, Gilles KA, Hamilton JK, Rebers P, Smith F. Colorimetric method for determination of sugars and related substances. Analytical chemistry. 1956;28(3):350-356.
  15. Wang K, Li W, Rui X, Chen X, Jiang M, Dong M. Structural characterization and bioactivity of released exopolysaccharides from Lactobacillus plantarum 70810. International journal of biological macromolecules. 2014;67:71-78.
  16. Nair VD, Panneerselvam R, Gopi R. Studies on methanolic extract of Rauvolfia species from Southern Western Ghats of India–In vitro antioxidant properties, characterisation of nutrients and phytochemicals. Industrial Crops and Products. 2012;39:17-25.
  17. Megala J, Geetha A. Free radical-scavenging and H+, K+-ATPase inhibition activities of Pithecellobium dulce. Food chemistry. 2010;121(4):1120-1128.
  18. Jayaprakasha G, Selvi T, Sakariah K. Antibacterial and antioxidant activities of grape (Vitis vinifera) seed extracts. Food research international. 2003;36(2): 117-122.
  19. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine. 1999;26(9-10):1231-1237.
  20. Zhu C, Zhai X, Li L, Wu X, Li B. Response surface optimization of ultrasound-assisted polysaccharides extraction from pomegranate peel. Food Chemistry. 2015;177:139-146.
  21. Tran TVT, Ho XAV, Le LS, Le TH, Nguyen THC, Nguyen CC, et al. Chemical structure of a novel heteroglycan polysaccharide isolated from the biomass of Ophiocordyceps Sobolifera. Journal of Molecular Structure. 2021;1232:129986.
  22. Kacurakova M, Capek P, Sasinkova V, Wellner N, Ebringerova A. FT-IR study of plant cell wall model compounds: pectic polysaccharides and hemicelluloses. Carbohydrate polymers. 2000;43(2):195-203.
  23. Surin S, Seesuriyachan P, Thakeow P, You S, Phimolsiripol Y. Antioxidant and antimicrobial properties of polysaccharides from rice brans. Chiang Mai J Sci. 2018;45 (3):1372-1382.
  24. Mohammed JK, Mahdi AA, Ahmed MI, Ma M, Wang H. Preparation, deproteinization, characterization, and antioxidant activity of polysaccharide from Medemia argun fruit. International journal of biological macromolecules 2020;155:919-926.
  25. Chaiklahan R, Srinorasing T, Chirasuwan N, Tamtin M, Bunnag B. The potential of polysaccharide extracts from Caulerpa lentillifera waste. International Journal of Biological Macromolecules. 2020;161:1021-1028.
  26. Xu X, Wu P, Wang T, Yan L, Lin M, et al. Synergistic effects of surfactant-assisted biodegradation of wheat straw and production of polysaccharides by Inonotus obliquus under submerged fermentation. Bioresource technology. 2019;278:43-50.
  27. Thambiraj SR, Phillips M, Koyyalamudi SR, Reddy N. Antioxidant activities and characterisation of polysaccharides isolated from the seeds of Lupinus angustifolius. Industrial Crops and Products. 2015;74:950-956.
  28. Tseng YH, Yang JH, Mau JL, Antioxidant properties of polysaccharides from Ganoderma tsugae. Food chemistry. 2008;107(2):732-738.
  29. Liu X, Bian J, Li D, Liu C, Xu S, Zhang G, et al. Structural features, antioxidant and acetylcholinesterase inhibitory activities of polysaccharides from stem of Physalis alkekengi L. Industrial Crops and Products. 2019;129:654-661.
  30. Chen H, Zeng J, Wang B, Cheng Z, Xu J, Gao W, et al. Structural characterization and antioxidant activities of Bletilla striata polysaccharide extracted by different methods. Carbohydrate Polymers. 2021;266:118149.
Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Copyright (c) 2023 Array