Isolation and quantification of major saponin ginsenoside Re from hairy root cultures of Panax vietnamensis Ha et Grushv by HPLC
Vol. 134 No. 1A (2025), Original Article
Vol. 134 No. 1A (2025)

Isolation and quantification of major saponin ginsenoside Re from hairy root cultures of Panax vietnamensis Ha et Grushv by HPLC

Original Article
https://doi.org/10.26459/hueunijns.v134i1A.7490
Published 2025-03-19
Tuan Vu Van
Faculty of Pharmacy, Phenikaa University, Hanoi, Vietnam
Thuy Trang Le
Faculty of Pharmacy, Phenikaa University, Hanoi, Vietnam
Ngan Le Thi
Faculty of Pharmacy, Phenikaa University, Hanoi, Vietnam
Thu Huong Nguyen Thi
Faculty of Pharmacy, Phenikaa University, Hanoi, Vietnam
My Lan Nguyen Thi
Faculty of Pharmacy, Phenikaa University, Hanoi, Vietnam
Anh Tuyet Trinh Thi
Faculty of Pharmacy, Phenikaa University, Hanoi, Vietnam
Quynh Truc Nguyen Thi
Faculty of Pharmacy, Phenikaa University, Hanoi, Vietnam
Thanh Tung Pham Ha
Faculty of Pharmacy, Phenikaa University, Hanoi, Vietnam
Huu Tung Nguyen
Faculty of Pharmacy, Phenikaa University, Hanoi, Vietnam
Mai Luong Khuat Thi
Molecular Biology Department, Agricultural Genetics Institute, Ha Noi, Vietnam
Hung Linh Le
Molecular Biology Department, Agricultural Genetics Institute, Ha Noi, Vietnam
Nguyen Ngoc Hieu*
Faculty of Pharmacy, Phenikaa University, Hanoi, Vietnam
PDF (Vietnamese)

Keywords

Sâm Việt Nam
rễ tóc
HPLC
ginsenoside Re Panax vietnamensis
hairy root
HPLC
ginsenoside Re

How to Cite

1.
Vũ Văn T, Lê TT, Lê Thị N, Nguyễn Thị TH, Nguyễn Thị ML, Trịnh Thị Ánh T, Nguyễn Thị QT, Phạm Hà TT, Nguyễn HT, Khuất Thị ML, Lê HL, Nguyễn Ngọc H. Isolation and quantification of major saponin ginsenoside Re from hairy root cultures of Panax vietnamensis Ha et Grushv by HPLC. hueuni-jns [Internet]. 2025Mar.19 [cited 2025Apr.26];134(1A):67-76. Available from: http://222.255.146.83/index.php/hujos-ns/article/view/7490
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Abstract

From the 80% ethanol extract of the hairy root cultures of Panax vietnamensis, a main dammarane-type saponin was isolated by using the chromatographic method. The isolate was identified as ginsenoside Re (1) via an extensive analysis by using NMR and MS, as well as comparison with published data. A quantitative procedure using HPLC with a UV detector has been developed to determine the ginsenoside Re content from the hairy roots of cultured P. vietnamensis, and the procedure was also validated according to the ICH guidelines in terms of accuracy, repeatability, linearity, and selectivity. The determination was carried out by using a C18 Shimadzu Shim-pack GIST (4.6 × 250 mm; 5 µm) column as a stationary phase with a gradient solvent system of acetonitrile-water as eluant. The flow rate was set at 1.0 mL/min and UV detection at 196 nm. This is the first report on the isolation and quantitative analysis of ginsenoside Re in the samples of P. vietnamensis hairy roots.

https://doi.org/10.26459/hueunijns.v134i1A.7490
PDF (Vietnamese)

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