Isolation and selection of Nitrobacter spp. from shrimp ponds in Hue City
Vol. 135 No. 1B (2026), Original Article
Vol. 135 No. 1B (2026)

Isolation and selection of Nitrobacter spp. from shrimp ponds in Hue City

Original Article
https://doi.org/10.26459/hueunijns.v135i1B.8466
Published 2026-06-29
Nguyen Thi Xuan Hong*
Faculty of Fisheries, University of Agriculture and Forestry, Hue Univeristy, Hue, Vietnam
Duc Quynh Anh Nguyen
Faculty of Fisheries, University of Agriculture and Forestry, Hue Univeristy, Hue, Vietnam
PDF

Keywords

ammonia oxidation
nitrifying bacteria
Nitrobacter bacteria
nitrite

How to Cite

1.
Xuan Hong NT, Nguyen DQA. Isolation and selection of Nitrobacter spp. from shrimp ponds in Hue City. hueuni-jns [Internet]. 2026Jun.29 [cited 2026Oct.1];135(1B):147-5. Available from: http://222.255.146.83/index.php/hujos-ns/article/view/8466
ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver

Download Citation

Endnote/Zotero/Mendeley (RIS) BibTeX
Crossref
Scopus
Google Scholar
Europe PMC

Abstract

Nitrite (NO2–) is a critical toxic intermediate in the nitrogen cycle and represents a major water quality concern in shrimp aquaculture. Elevated nitrite concentration can severely compromise shrimp health, productivity, and survival. This study aimed to isolate and identify Nitrobacter spp. with potential applicability in the bioremediation of nitrite-contaminated aquaculture systems. Seven Nitrobacter spp. strains were successfully isolated from sediment samples collected from shrimp ponds. The isolates exhibited consistent biochemical characteristics, among which PH11 showed a higher growth rate in vitro than the other isolates. Furthermore, this strain demonstrated efficient nitrite removal at NaCl concentrations of 0–2.5% and within a pH range of 6.5–8.0. Collectively, these findings suggest that Nitrobacter sp. PH11 is a highly promising candidate for nitrite remediation in freshwater or low-salinity aquaculture systems. Overall, a thorough understanding of nitrite toxicity and proper management of nitrogen cycling in aquatic environments are key issues to promoting the health, performance, and sustainable production of cultured species.

https://doi.org/10.26459/hueunijns.v135i1B.8466
PDF

References

  1. Ha TL, Anh PT, Thanh NT. Isolation and selection of nitrifying bacterial strains for application in water pollution treatment. Journal of Science and Technology. 2007;45(3):95-100.
  2. Ge J, Zhang Y, Heo YJ, Park SJ. Advanced design and synthesis of composite photocatalysts for the remediation of wastewater: A review. Catalysts. 2019;9(2):122.
  3. Tampangallo BR, Idra Jaya Asaad A, Undu MC, Khairana Kadriah IA, Anshary H. Bacterial diversity in superintensive vannamei shrimp aquaculture wastewater treatment plants in Barru and Takalar Regencies. IOP Conference Series: Earth and Environmental Science. 2020;564(1):012007.
  4. Iber BT, Kasan NA. Recent advances in Shrimp aquaculture wastewater management. Heliyon. 2021;7(11):e08283.
  5. Iber BT, Okomoda VT, Rozaimah SA, Kasan NA. Eco-friendly approaches to aquaculture wastewater treatment: Assessment of natural coagulants vis-a-vis chitosan. Bioresource Technology Reports. 2021;15:100702.
  6. Yusoff FM, Banerjee S, Khatoon H, Shariff M. Biological approaches in management of nitrogenous compounds in aquaculture systems. Dynamic biochemistry, process biotechnology and molecular biology. 2011;5(1):21-31.
  7. Kroupova H, Machova J, Svobodova Z. Nitrite influence on fish: a review. Veterinarni medicina-praha-. 2005;50(11):461-471.
  8. Huong DTT, Thu CCM. The effects of nitrite on the molting cycle and growth of freshwater prawn (Macrobrachium rosenbergii). CTU Journal of Science. 2012;21b:19-28.
  9. Robles‐Porchas GR, Gollas‐Galván T, Martínez‐Porchas M, Martínez‐Cordova LR, Miranda‐Baeza A, Vargas‐Albores F. The nitrification process for nitrogen removal in biofloc system aquaculture. Reviews in Aquaculture. 2020;12(4):2228-49.
  10. Kim JK, Park KJ, Cho KS, Nam SW, Park TJ, Bajpai R. Aerobic nitrification–denitrification by heterotrophic Bacillus strains. Bioresource Technology. 2005;96(17):1897-906.
  11. Nogueira R, Melo LF. Competition between Nitrospira spp. and Nitrobacter spp. in nitrite‐oxidising bioreactors. Biotechnology and bioengineering. 2006;95(1):169-75.
  12. Ma S, Zhang D, Zhang W, Wang Y. Ammonia stimulates growth and nitrite-oxidising activity of Nitrobacter winogradskyi. Biotechnology & Biotechnological Equipment. 2014;28(1):27-32.
  13. Aleem MI, Alexander M. Nutrition and physiology of Nitrobacter agilis. Applied Microbiology. 1960;8(2):80-4.
  14. Kim DD, Nam NV, Chi LT. Investigation and assessment of the current status of medicines, chemicals, and biological preparations used in aquaculture to propose management solutions. Summary report. Research Institute for Aquaculture No.1; 2006.
  15. Gram C. The differential staining of Schizomycetes in tissue sections and in dried preparations. Fortschitte der Medicin. 1884;2(6):185-9.
  16. Boon N, De Windt W, Verstraete W, Top EM. Evaluation of nested PCR–DGGE (denaturing gradient gel electrophoresis) with group-specific 16S rRNA primers for the analysis of bacterial communities from different wastewater treatment plants. FEMS microbiology ecology. 2002;39(2):101-12.
  17. Dung TN. Molecular biology practical handbook. Can Tho: Can Tho University Publishing House; 2011. 175 p.
  18. Degrange V, Bardin R. Detection and counting of Nitrobacter populations in soil by PCR. Applied and Environmental Microbiology. 1995;61(6):2093-8.
  19. Hiep HP, Minh TTN, Nga HTT. Isolation and selection of indigenous high-activity ammonia-oxidising bacteria in Quang Ninh and Hai Phong. TNU Journal of Science and Technology. 2021;226(14),30-36.
  20. Bothe H, Ferguson S, Newton WE, editors. Biology of the Nitrogen Cycle: COST edition. Elsevier; 2006.
  21. Vu TQ, Ut VN. Water Quality for Aquaculture (Original work by Claude E. Boyd, Auburn University, Alabama 36849, USA). Can Tho: Department of Fisheries Management and Exploitation, Can Tho University. 2014. 63 p.
  22. Chen JC, Chin TS. Acute toxicity of nitrite to tiger prawn, Penaeus monodon, larvae. Aquaculture. 1988;69(3-4):253-62.
  23. Lin YC, Chen JC. Acute toxicity of nitrite on Litopenaeus vannamei (Boone) juveniles at different salinity levels. Aquaculture. 2003;224(1-4):193-201.
  24. US Department of Health and Human Services. Agency for Toxic Substances and Disease Registry-ATSDR. US Department of Health and Human Services.
  25. Boyd CE, Tucker CS. Pond aquaculture water quality management. Springer Science & Business Media; 2012.
  26. Gale EF. Factors influencing the enzymic activities of bacteria. Bacteriological reviews. 1943;7(3):139-73.
  27. García-Heredia A. Plasma membrane-cell wall feedback in bacteria. Journal of Bacteriology. 2023;205(3):e00433-22.
  28. Baker-Austin C, Dopson M. Life in acid: pH homeostasis in acidophiles. Trends in microbiology. 2007;15(4):165-71.
  29. Jin Q, Kirk MF. pH as a primary control in environmental microbiology: 2. Kinetic perspective. Frontiers in Environmental Science. 2018;6:101.
  30. Morrissey EM, Gillespie JL, Morina JC, Franklin RB. Salinity affects microbial activity and soil organic matter content in tidal wetlands. Global change biology. 2014;20(4):1351-62.
  31. Crawford RE, Allen GH. Seawater inhibition of nitrite toxicity to chinook salmon. Transactions of the American Fisheries Society. 1977;106(1):105-9.
  32. Mazik PM, Hinman ML, Winkelmann DA, Klaine SJ, Simco BA, Parker NC. Influence of nitrite and chloride concentrations on survival and hematological profiles of striped bass. Transactions of the American Fisheries Society. 1991;120(2):247-54.
Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Copyright (c) 2026 Array