http://222.255.146.83/index.php/hujos-ns/issue/feed Hue University Journal of Science: Natural Science 2024-09-30T00:00:00+00:00 Hue University Journal of Science: Natural Science tckh_dhh@hueuni.edu.vn Open Journal Systems <p><strong>ISSN (Print) 1859-1388</strong></p> <p><strong>ISSN (Online) 2615-9678</strong></p> <p><strong>DOI: 10.26459</strong></p> <p><strong>Editor in chief: </strong>Do Thi Xuan Dung</p> <p><strong>HUJOS-NS Editor: </strong>Duong Tuan Quang</p> <p><strong>Managing Editor: </strong>Tran Xuan Mau</p> <p><strong>Editorial Assistant: </strong>Duong Duc Hung</p> <p><strong>Phone:</strong> 02343845658 | <strong>Email: </strong>ddhung@hueuni.edu.vn</p> <p><strong>Indexing: <a href="https://asean-cites.org/aci_search/journal.html?b3BlbkpvdXJuYWwmaWQ9MTE1MDI" target="_blank" rel="noopener">ACI (ASEAN CITATION INDEX)</a>; <a href="https://doaj.org/toc/2615-9678" target="_blank" rel="noopener">DOAJ (Directory of Open Access Journals)</a></strong></p> http://222.255.146.83/index.php/hujos-ns/article/view/7102 Isolation of endogenous bacteria from wild orchids 2023-05-18T01:41:24+00:00 Huu Tho Nguyen nguyenhuutho@hueuni.edu.vn Thị Bích Phượng Trương ttbphuong@hueuni.edu.vn Thị Xuân Tuý Hồ hothixuantuy@hueuni.edu.vn Thành Phạm efphamthanh@gmail.com Thị Kim Cúc Nguyễn ntkcuc.huib@hueuni.edu.vn <p>Endophytic bacteria reside in plant tissues without causing damage to the host and enhance plant growth under both normal and adverse conditions. In this study, 30 strains of endophytic bacteria were isolated from different wild orchids, including <em>Dendrobium anosmum</em>, <em>Aerides falcata</em>, <em>Dendrobium thyrsiflorum</em>, <em>Dendrobium lindleyi,</em> and <em>Cymbidium finlaysonianum</em>. The colony morphology of the isolated strains was mainly white (73.4%), and slimy (60%), with regularly rounded edges (63.3%), a few had a dry or glossy surface and a wavy edge. The results of biochemical characterization studies showed that 18/30 strains could decompose phosphorus; 15/30 strains could produce producing IAA; 16/30 strains could fix nitrogen; and 18/30 strains were likely to generate NH<sub>3</sub>. Among them, three isolated strains, named L4, L6, and C4, as outstanding characteristics than other strains, were selected for identification and identified as <em>Burkholderia </em>sp.<em>, Bacillus cereus</em>, and <em>Pseudomonas</em> <em>boreopolis</em>, respectively. These microbes are potential strains that can be used as microbial products for agricultural applications, especially in growing orchid seedlings.</p> 2024-10-09T00:00:00+00:00 Copyright (c) 2024 Hữu Thọ Nguyễn, Thị Bích Phượng Trương, Thị Xuân Tuý Hồ, Thành Phạm, Thị Kim Cúc Nguyễn http://222.255.146.83/index.php/hujos-ns/article/view/7301 Establishment of undergroundwater quality index (GWQI) and application to Hai Lang district, Quang Tri province 2023-10-09T06:33:53+00:00 Kiên Trương Trung truongtrungkien72@gmail.com Thị Thanh Tuyền Mai thanhtuyenmtqt@gmail.com Quý Tùng Trương truongquytung@gmail.com Văn Hợp Nguyễn ngvanhopkh@gmail.com <p>In this study a Ground Water Quality Index (GWQI) was established and applied to Hai Lang district in Quang Tri province. Ten basic water quality parameters selected for establishment of the GWQI consisted of: pH, TDS, hardness (HARD); SO4, COD, N-NH4, N-NO3, Fe, Mn and Total coliform (TC). Principal component analysis (PCA) was applied to the monitoring data set of the period 2015-2018 in the district to determine the weights (w<sub>i</sub>) of the parameters. The linear sub-index functions (q<sub>i</sub>) were set up basing on the allowable limits of the parameters specified in the National Technical Regulation on Domestic Water Quality (QCVN 01-1:2018/BYT) issued by the Ministry of Health. Three (3) different methods were used to calculate index GWQI<sub>M</sub> (multiplicate form), GWQI<sub>A</sub> (additive form) and GWQI<sub>Ref</sub> (reference form) for the water quality data set. The indexes were all funtions of sub-index q<sub>i</sub> and weights w<sub>i</sub>. The obtained results show that the index GWQI<sub>M</sub> is more suitable than GWQI<sub>A</sub> and GWQI<sub>Ref</sub> in reflecting the groundwater quality. Applying the GWQI<sub>M</sub> to the water quality data set of the period 2019 - 2021 in the district indicated that there are 89% of GWQI<sub>M</sub> values classified as Very good or Good water quality, 7% as Medium 1% as Poor and 3% as Very poor (or Unfit for drinking).</p> 2024-10-09T00:00:00+00:00 Copyright (c) 2024 Kiên Trương Trung, Văn Hợp Nguyễn, Quý Tùng Trương, Thị Thanh Tuyền Mai http://222.255.146.83/index.php/hujos-ns/article/view/7316 Simultaneous determination of Amlodipine and Atovastatin in a multicomponent drug using infrared spectroscopy coupled with multivatiate analysis 2023-09-30T02:47:11+00:00 Duy Luu Nguyen ndluu@dhktyduocdn.edu.vn Nguyen Thi Thanh Tram ntttram@gmail.com Pham Phu Quoc ppquoc@gmail.com Nguyễn Hùng Nhật Duy nhnduy@gmail.com Nguyễn Đình Luyện ndluyen@hueuni.edu.vn Trần Thúc Bình ttbinh@hueuni.edu.vn <p>The development and validation of a UV spectroscopy method combined with multivariate analysis methods including classical least squares, principal component regression, and partial least squares were performed to simultaneously quantify Amlodipine (AML) and Atorvastatin (ATO). A central composite design experimental method was used to construct calibration and validation sets. UV absorption spectra were measured in the wavelength range of 230-260 nm. Multivariate regression methods were constructed based on the measured spectra of the calibration and validation sets, resulting in: R<sup>2</sup> &gt; 0.9927, RMSEC<sub>AML</sub> &lt; 1 ppm, RMSEC<sub>ATO</sub> &lt; 2 ppm, and relative prediction error (REP) &lt; 5%. The recovery ranged from 99.20% - 108.13% for AML and 99.10% - 107.19% for ATO for all three methods. The repeatability (RSD) was smaller than the Horwitz value. ANOVA analysis compared the mean values of the methods with the HPLC method, and no significant differences were found. The simultaneous quantification of AML and ATO in Zoamco-a tablets resulted in AML and ATO content within the range of 90-110% of the labeled content, which complies with the regulations of the Vietnamese Pharmacopoeia V.</p> 2024-09-30T00:00:00+00:00 Copyright (c) 2024 Duy Lưu Nguyễn http://222.255.146.83/index.php/hujos-ns/article/view/7320 Isolation and evaluation of antioxidants from leaves of Uvaria rufa (Dunal) Blume – Annonaceae 2023-10-30T14:10:39+00:00 Thị Minh Tâm Trần ttmtam@ttn.edu.vn Ngọc Thuỵ Huỳnh huynhthuyth@gmail.com <p><em>Uvaria rufa </em>(Dunal) Blume is exclusively utilized for postpartum women in certain traditional medicine hospitals. This study was carried out to isolate the compounds with antioxidant effects in the leaves to use this medicinal plant more effectively. Extraction, seperation, isolation and purification were done as routine work. Structure elucidation was based on ultraviolet spectroscopy, mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy methods. The <em>in vitro</em> antioxidant activity of isolated compounds was performed on DPPH (2,2- diphenyl-1-picrylhydrazyl) free radical quenching method on 96 well plates. From ethyl acetate fraction, 2 compounds, ie. isoquercitrin and rutin were isolated, exhibited antioxidant activity with IC<sub>50</sub> values of 3.33 µM and 16.42 µM, respectively. These results will be the basis for further testing of other biological activities of the plant.</p> 2024-09-30T00:00:00+00:00 Copyright (c) 2024 Tran Thi Minh Tam*, Huynh Ngoc Thuy http://222.255.146.83/index.php/hujos-ns/article/view/7328 Evaluation of Antibacterial Activity Properties of Carbon Quantum Dots Synthesized from Orange Juice 2024-03-21T02:22:04+00:00 Thị Bảo Chi Lê ltbchi@huemed-univ.edu.vn Thị Kim Dung Lê ltkdung.cb@huemed-univ.edu.vn Thị Thủy Ung utthuy@huemed-univ.edu.vn Hoàng Tùng Đỗ dhtung@iop.vast.vn Duy Quỳnh Như Trần tdqnhu@huemed-univ.edu.vn Minh Hoa Nguyễn nguyenminhoa@hueuni.edu.vn <p>In this study, carbon quantum dots (CDs) were synthesized through plasma processing of orange juice at atmospheric pressure and low temperature. The resulting CDs exhibited an average size of approximately 4.7 nm with a narrow distribution. A blue emission was observed with a peak wavelength of 460 nm under excitation at 390 nm. The formation of CDs was confirmed by UV-Vis absorption spectra and Raman scattering analyses. The antibacterial activities of the CDs were evaluated against <em>Staphylococcus aureus </em>ATCC 29213 and <em>Escherichia coli</em> ATCC 25922 strains. Results indicated that inhibitory effects on bacterials strains were observed after 30 seconds of exposure for <em>Escherichia coli</em> ATCC 25922 and 5 minutes for <em>Staphylococcus aureus </em>ATCC 29213. The average antibacterial efficiency of the CDs sample against <em>Staphylococcus aureus</em> and <em>Escherichia coli</em> strains was found to be 62.1% and 57.0%, respectively.</p> 2024-09-30T00:00:00+00:00 Copyright (c) 2024 Le Thi Bao Chi, Le Thi Kim Dung, Ung Thi Thuy, Do Hoang Tung, Tran Duy Quynh Nhu, Nguyen Minh Hoa* http://222.255.146.83/index.php/hujos-ns/article/view/7359 Extraction and isolation of compounds from chloroform extract of Goniothalamus donnaiensis leaves, Annonaceae 2023-12-13T14:00:47+00:00 Thi Thu Hong Lê hongle@lhu.edu.vn <p>Two compounds including a stylryl lactone: goniodiol, and a phytosterol: daucosretol were isolated from the chloroform extract of the leaves of <em>Goniothalamus donnaiensis</em>. Leaves of <em>G. donnaiensis</em> were collected in Vinh Cuu district, Dong Nai province in November 2019. Their chemical structures were elucidated by ESI-mass, 1 H- NMR, 13 C-NMR, DEPT, HSQC and HMBC spectra. These two compounds were first isolated from the leaves of <em>Goniothalamus donnaiensis</em>.</p> 2024-09-30T00:00:00+00:00 Copyright (c) 2024 Le Thi Thu Hong* http://222.255.146.83/index.php/hujos-ns/article/view/7401 Cloning and expression nahC gene encoding 1,2-dihydroxynaphthalene dioxygenase of Paenibacillus naphthalenovorans 4B1 2024-03-28T21:33:10+00:00 Le Thi Ha Thanh lethihathanh@hueuni.edu.vn Thị Ái Thoa Nguyễn mynguyencalo1707@gmail.com Thị Xuân Nguyễn xuannguyen20111998@gmail.com Vũ Ngọc Thi Trần tvngocthi@husc.edu.vn <p>Polycyclic aromatic hydrocarbon compounds used in many industries have caused environmental pollutions and left consequences to human health. Naphthalene, the simplest polycyclic aromatic hydrocarbon, is widely used as a model compound for biodegradation studies in bacteria. The 1,2-dihydroxynaphthalene dioxygenase, a benzene ring-cleavage enzyme, converts intermediate compounds in the naphthalene degradation pathway into less toxic compounds. In this study, the <em>nahC </em>gene encoding 1,2-dihydroxynaphthalene dioxygenase from the moderate thermophilic <em>Paenibacillus naphthalenovorans </em>strain 4B1 was cloned into pGEM-T Easy vector. Subsequently, the gene after confirming the correct DNA sequence was ligated into pCold<sup>TM</sup> II expression vector and transformed to <em>Escherichia coli</em> BL21. The SDS-PAGE results showed that the <em>nahC </em>gene was successfully expressed in <em>E. coli</em> BL21 with 0.5 mM IPTG. The molecular weight of resulting recombinant enzyme was approximately 35 kDa. These results are the basis for enzyme purification and characterization.</p> 2024-09-30T00:00:00+00:00 Copyright (c) 2024 Le Thi Ha Thanh*, Nguyen Thi Ai Thoa, Nguyen Thi Xuan, Tran Vu Ngoc Thi http://222.255.146.83/index.php/hujos-ns/article/view/7417 Antioxidant activity of collagen/chitosan/ Archidendron clypearia extract composite materials 2024-01-24T14:04:38+00:00 Ngọc Nguyễn Hoàng Lương ngocnhl@huit.edu.vn Thi Trần Thị Văn ttvthi@hueuni.edu.vn Sơn Lê Lâm lelamson@hueuni.edu.vn Nhung Nguyễn Minh nhungnguyen.hc@gmail.com Mẫn Nguyễn Quang nqman.dhyd@hueuni.edu.vn Vũ Hồ Xuân Anh anhvu2303@gmail.com Minh Trần Thanh tranthanhminh@hueuni.edu.vn Thắng Nguyễn Việt nguyenvietthang@hueuni.edu.vn Như Nguyễn Thị nhunguyen14107@gmail.com Hạnh Nguyễn Thị Hồng nthhanh12a2@gmail.com Hiếu Lê Trung lthieu@hueuni.edu.vn <p>In this study, we have synthesized a new material with good antioxidant activity. For the first time, <em>Archidendron clypearia</em> extract was included in the collagen/chitosan/TEOS mixture, the resulting material had antioxidant capacity evaluated through DPPH and ABTS radical scavenging and the total antioxidant capacity method, with low IC<sub>50</sub> values (IC<sub>50</sub> = 43.60 μg/mL and 46.57 μg/mL for DPPH and ABTS radical scavenging capacity) and the total antioxidant capacity was 83.06 ± 0.56 mg GA/g. The total content of phenolic and flavonoid compounds in Coll-Cs-TEOS-MD 0.5 is 36.87 ± 0.18 mg GA/g and 28.12 ± 0.15 mg QE/g, respectively. Material characteristics are examined through infrared spectroscopy (IR) and scanning electron microscope (SEM). Coll-Cs-TEOS-MD 0.5 material shows good antioxidant activities, which is very promising as a biocompatible material for biomedical applications.</p> 2024-09-30T00:00:00+00:00 Copyright (c) 2024 Nguyen Hoang Ngoc Luong, Tran Thi Van Thi, Le Lam Son, Nguyen Minh Nhung, Nguyen Quang Man, Ho Xuan Anh Vu, Tran Thanh Minh, Nguyen Viet Thang, Nguyen Thi Nhu, Nguyen Thi Hong Hanh, Le Trung Hieu* http://222.255.146.83/index.php/hujos-ns/article/view/7426 Synthesis and photocatalytic activity of ZnO/biochar composite for the degradation of doxycycline under visible light irradiation 2024-03-01T03:58:25+00:00 Văn Hưng Nguyễn Nguyenvanhung@dthu.edu.vn Thanh Tươi Nguyễn nguyentuoi1982@gmail.com Thị Minh Nguyệt Bùi btmnguyet@dthu.edu.vn hanh Tuyền Lê thanhtuyenle1980@gmail.com Hải Phong Nguyễn nhphong@hueuni.edu.vn Thị Hòa Lê lethihoa@hueuni.edu.vn <p>In this study, ZnO/biochar (ZBC) materials were successfully synthesized by simple hydrolysis of a mixture of Zn(NO<sub>3</sub>)<sub>2</sub> salt and biomass derived from reed stems. The synthesized materials were characterized by XRD, SEM, TEM, BET, FT-IR and UV-Vis-DRS methods. The photocatalytic degradation of doxycycline (DC) was studied from the influencing factors of pH and concentration. Characterization shows that ZnO is well dispersed on the biochar surface. The presence of biochar helped improve the specific surface area, narrow the band gap and enhance the optical absorption in the visible light region of ZnO. The ZBC composite at ZnO/biochar mass ratio = 1/0.25 showed the highest photodegradation efficiency, about 3.5 times higher than pure ZnO in DC decomposition. Kinetic studies showed that DC degradation followed an apparent first-order kinetic model with rate constant k<sub>app</sub> = 2.03×10<sup>-2</sup> min<sup>-1</sup> at DC concentration of 9.0 mg/L. Research shows that ZnO/biochar material has great potential in treating antibiotic-contaminated wastewater.</p> 2024-09-30T00:00:00+00:00 Copyright (c) 2024 Nguyen Van Hung, Nguyen Thanh Tuoi, Bui Thi Minh Nguyet, Le Thanh Tuyen, Nguyen Hai Phong, Le Thi Hoa* http://222.255.146.83/index.php/hujos-ns/article/view/7430 Research on enhancing marine microalgae biomass Tetraselmis sp. for nitrogen and phosphorus removal from brakish-salt water rich in nutrients 2024-03-04T20:54:38+00:00 Thanh Lộc Đặng Thị dangthithanhloc@hueuni.edu.vn Le Van Tuan levantuan@hueuni.edu.vn Thu Lien Nguyen Thi nttliencnsh@hueuni.edu.vn Phúc Lộc Nguyễn Công locnguyencongphuc@gmail.com Tung Truong Quy truongquytung@gmail.com Tri Ta Ngoc ngoctri2810@gmail.com Chung Duong Thanh thanhchung@hueuni.edu.vn Phong Phan Hai phphong@hueuni.edu.vn <p>This study investigates the cultivation of <em>Tetraselmis</em> sp., a marine microalgae species, in seawater enriched with elevated levels of nutrient salts (N, P). The primary objective was to determine the suitable growth conditions for <em>Tetraselmis</em> sp., considering variations in nutrient composition, pH levels, initial inoculum density, and differing initial TN:TP ratios. Results indicate robust growth of <em>Tetraselmis</em> sp. in F/2 seawater medium with a salinity of 30 ‰ and pH 8, with initial inoculum densities ranging from 5% to 10%. <em>Tetraselmis</em> sp. exhibited strong growth potential in environments with elevated nutrient salt concentrations (N, P). Specifically, as TN concentration increased from 9.7 to 56.3 mg/L and TP concentration rose from 0. 1 to 3.7 mg/L, cell density increased from 6.98×10<sup>5</sup> (cells/mL) to 1.34×10<sup>6</sup> (cells/mL) after a 15-day cultivation period. Total phosphorus treatment efficiency ranged from 82% to 100%. Total nitrogen treatment efficiency ranged from 97% to 83% for initial T-N concentrations of 10 to 25 mg/L. However, with higher T-N concentrations (~56 mg/L), the treatment efficiency reached only 58% after 15 days of cultivation. These findings highlight the significant potential of cultivating <em>Tetraselmis </em>sp. for N and P removal from brackish-salt water rich in nutrients in Vietnam.</p> 2024-09-30T00:00:00+00:00 Copyright (c) 2024 Dang Thi Thanh Loc, Le Van Tuan, Thu Lien Nguyen Thi, Nguyen Phuc Cong Loc, Tung Truong Quy, Tri Ta Ngoc, Chung Duong Thanh, Phong Phan Hai http://222.255.146.83/index.php/hujos-ns/article/view/7431 The Spin-Coater, Hydrothermal Synthesis and Characterizations of MoS2/TiO2 Nanoflake for Photoelectrochemical Application 2024-03-29T10:59:35+00:00 Nguyễn Tiến Đại nguyentiendai@duytan.edu.vn Nguyễn Văn Mạnh nguyenvanmanh@haui.edu.vn Lâm Văn Năng lvnang@hluv.edu.vn Hoàng Trọng Phúc htphuc90@gmail.com Lê Văn Công Hậu levanconghau_t66@hus.edu.vn Bùi Văn Ban buivanban_t66@hus.edu.vn <p>MoS<sub>2</sub>/TiO<sub>2</sub> nanoflakes (NFs) have been synthesized using the spin-coater and hydrothermal method at a temperature of 180 <sup>o</sup>C for photoelectrochemical application. The thick-MoS<sub>2</sub> nanoflakes are about a few nano to tens of nano for 4 – 8 h, which consist of highly active sites at edges and planes to improve photoconversion efficiency. XRD pattern of MoS<sub>2</sub>/TiO<sub>2</sub> NFs showed both hexagonal and anatase phases for MoS<sub>2</sub> and TiO<sub>2</sub>, respectively, with a high crystal quality. At room temperature, the photoluminescence spectra of MoS<sub>2</sub> NFs exhibited an asymmetric shape and good emission peak at 688 nm. The photoelectrochemical cell of the MoS<sub>2</sub>/TiO<sub>2 </sub>nanostructure obtained a photocurrent density of 3.5 mA<em>.</em> cm<sup>–2</sup> at –0.8 V (vs. SCE), using 0.5 M H<sub>2</sub>SO<sub>4</sub> electrolyte. As a result, we propose a potential fabrication of high-efficiency optoelectronic devices through the synthesis of MoS<sub>2</sub> NFs hybrid with traditional photocatalyst oxide materials by the one-step hydrothermal approach.</p> 2024-09-30T00:00:00+00:00 Copyright (c) 2024 Nguyen Tien Dai*, Nguyen Van Manh, Lam Van Nang, Hoang Trong Phuc, Le Van Cong Hau, Bui Van Ban http://222.255.146.83/index.php/hujos-ns/article/view/7455 Synthesis of nano materials on the base of rice husk ash and CeO2 for application as a growth stimulant for bell peeppers plan (capsicum annuum L.) 2024-04-22T06:17:26+00:00 Huỳnh Xuân Trường hxtruong2703@gmail.com <p>In this study, CeO<sub>2</sub>/SiO<sub>2</sub> material was synthesized and applied as a growth stimulant for bell pepper plants from Ce(NO<sub>3</sub>)<sub>4</sub> precursor and rice husk ash using gamma irradiation method. Material morphology and structure were confirmed through physicochemical characterization methods including: XRD, EDX, IR and SEM characterization. The results of surveying the effects of CeO<sub>2</sub>/SiO<sub>2</sub> materials on the growth and development of bell pepper plants show that CeO<sub>2</sub>/SiO<sub>2</sub> significantly affects the growth and development of bell pepper plants. Experimental data obtained showed that bell pepper yield increased by 22 tons/ha, average root length increased by 3.6 cm and harvest time was 11 days less than the control sample. In addition, bell pepper plants supplemented with CeO<sub>2</sub>/SiO<sub>2</sub> are stronger and do not have yellow leaves. This shows that CeO<sub>2</sub>/SiO<sub>2</sub> has a growth stimulating effect on bell pepper plants.</p> 2024-09-30T00:00:00+00:00 Copyright (c) 2024 Huynh Xuan Truong, Nguyen Viet Xuan, Nguyen Thuy Nhi, Pham Tran Thanh Ngan, Dang Thi Dieu Linh, Le Thi Thu Huong, Cao Van Hoang* http://222.255.146.83/index.php/hujos-ns/article/view/7544 A Sexual size difference and microhabitat use of Amolops ricketti group (Anura, Ranidae) in Bach Ma national park, Thua Thien Hue province, Vietnam 2024-07-26T08:36:15+00:00 Đức Lợi duongducloi@dhsphue.edu.vn <p><em>Amolops ricketti</em> (group) is a species with nutritional and medicinal value. However, little is known about sexual size dimorphism (SSD), microhabitat use, and the mode of action of this species. We observed the behavior of this species in the field at Bach Ma National Park, combined with analyzing morphological characteristics in the laboratory. Based on 97 adult Rock Frog samples (44 males and 53 females), assessments and comparisons of morphological measurements and calculations of the SSD index were performed. The results showed that the average trunk length (SVL) of adult females was longer than that of adult males, with an SSD index of 0.23. This species lives in fast-flowing stream water, also known as torrential environments, with microhabitats mainly on high-slope rocks dominating (52.58%), followed by rocks with low-slope (34.02%). This species used the highest proportion of time for lying and waiting activities (77.78%). The mode of activity of this species is more suitable for the sit-and-wait model than the wide forager model. Some environmental, physical, and chemical indicators in the species' living area were also surveyed in this study.</p> 2024-09-30T00:00:00+00:00 Copyright (c) 2024 Duong Duc Loi*, Nguyen Hai Trieu, Dang Thi Ngoc, Tran Van Giang http://222.255.146.83/index.php/hujos-ns/article/view/7380 Molecular detection methods for Pasteurella multocida and Mannheimia haemolytica 2023-12-12T02:33:57+00:00 Cong Tuan Le lctuan@hueuni.edu.vn Thi Xuan Tuy Ho hothixuantuy@hueuni.edu.vn Thi Dieu The Nguyen ntdthe@hueuni.edu.vn Thi Thu Thao Nguyen nttthao.huib@hueuni.edu.vn Thi Ngoc Han Hoang hoanghan158@gmail.com Thi Diem Nguyen ntdiem@hueuni.edu.vn Van Phu Nguyen nguyenvanphu@hueuni.edu.vn Huu Tho Nguyen nguyenhuutho@hueuni.edu.vn Thi Oanh Nguyen nguyenthioanh@hueuni.edu.vn Thi Kim Cuc Nguyen ntkcuc.huib@hueuni.edu.vn Xuan Huy Nguyen nguyenxuanhuy@hueuni.edu.vn <p><em>P. multocida and M. haemolytica </em>are the main bacteria causing pasteurellosis in household animals, leading to extensive economic losses for the livestock industry<em>. </em>Different traditional methods have been used since the 1980s to detect<em> P. multocida </em>and<em> M. haemolytica </em>infection<em>. </em>Although these methods help diagnose pasteurellosis in the early stages, they are limited in sensitivity and specificity. Therefore, modern diagnostic methods that rely on bacterial genomics to improve sensitivity, specificity, and stability have developed. This report is based on our team’s results and published papers related to diagnosis, typing, and polymorphism of pasteurellosis via PCR, Multiplex PCR, real-time PCR, and LAMP and analyze the advances and drawbacks of each method to provide a comprehensive view of pasteurellosis diagnosis.</p> 2024-09-30T00:00:00+00:00 Copyright (c) 2024 Le Cong Tuan, Ho Thi Xuan Tuy, Nguyen Thi Dieu The, Nguyen Thi Thu Thao, Hoang Thi Ngoc Han, Nguyen Thi Diem, Nguyen Van Phu, Nguyen Huu Tho, Nguyen Thi Oanh, Nguyen Thi Kim Cuc, Nguyen Xuan Huy http://222.255.146.83/index.php/hujos-ns/article/view/7312 Study on reducing power of Morinda citrifolia L. Fruit Extract (McFE) as a reduced agent in the green synthesis of gold nanoparticles 2023-11-03T09:04:47+00:00 Dang Xuan Du dangxuandu@sgu.edu.vn Thi Ngoc Thuy Nguyen ngocthuy067240@gmail.com Thi Minh Man Tran tranthiminhman1981@gmail.com Duc Tien Trinh trinhductien.hungvuong@gmail.com <p>The reducing power of Morinda citrifolia L. Fruit Extract (McFE) for the green synthesis of gold nanoparticles was evaluated by ferricyanide method, infrared spectroscopy (FT-IR) and ABTS•+ free radical scavenging activity. The results showed that the extract sample at the concentration of 75 mg/mL (McFE75) stored for 2 days gave the highest reducing power. FTIR spectroscopy indicated the involvement of carboxyl, amine and hydroxyl groups in the the composition of McFE75. Gold nanoparticles (AuNPs) were green synthesized using McFE75 as a reducing agent with a volume ratio of McFE75/Au<sup>3+</sup> 1 mM of 1:10. The UV-vis spectroscopy, TEM and SEM methods were used to monitor the formation and morphology of AuNPs. The Zeta potential method was used to evaluate the stability of the gold colloidal system. The results showed that the time of 110 minutes was suitable to fabricate the gold nano colloidal system at room temperature. The size of obtained AuNPs was of about 17 nm, Zeta potential = -14.2 mV. The obtained nano gold colloidal system was quite stable. After 2.5 months of storage, the size and Zeta potential of AuNPs were 26 nm and -6.5 mV, respectively.</p> 2024-09-30T00:00:00+00:00 Copyright (c) 2024 Dang Xuan Du, Nguyen Thi Ngoc Thuy, Tran Thi Minh Man, Trinh Duc Tien http://222.255.146.83/index.php/hujos-ns/article/view/7458 In vitro and in silico studies of compounds extracted from Elsholatzia cillata against bacteria Streptococcus pneumoniae and Streptococcus pyogenes 2024-05-28T14:29:46+00:00 Vĩnh Phú Nguyễn nvphu.dhyd@hueuni.edu.vn Hoàng Bách Võ vohoangbach3103@gmail.com Thị Phương Nga Hoàng hoangngahll520@gmail.com Quang Huy Trần quanghuymt27@gmail.com Đại Châu Nguyễn nguyendaichau183599@gmail.com Thị Ái Nhung Nguyễn ntanhung@hueuni.edu.vn Tứ Quý Phan phantuquy@ttn.edu.vn Thị Thanh Hải Nguyễn nguyenthanhhai@hueuni.edu.vn <p>The<em> Elsholatzia cillata </em>essential oil was extracted and characterized by gas chromatography-mass spectrometry (GC-MS) for compositional specificity. GC-MS analysis revealed the presence of 13 natural components in the essential oil (<strong>E1 - E13</strong>). The ability of active components that inhibit <em>Streptococcus pneumoniae</em> with 5XU1 protein and <em>Strepcococcus pyogene</em> with 4XCH protein was identified using <em>in vitro</em> and <em>in silico</em> studies. Evaluating the antibacterial ability of compounds in the<em> Elsholatzia cillata </em>essential oil under <em>in vitro</em> conditions that against <em>S. pneumoniae</em> and <em>S. pyogenes</em> exhibited that the results of inhibition zone diameter and minimum concentration inhibition (MIC) was 45 mm and 0.15625 µL.mL<sup>-1</sup> on <em>S.pneumoniae</em> strain and 34 mm and 0.15625 µL.mL<sup>-1</sup> on <em>S. pyogenes</em> strain, respectively. Molecular docking simulations confirmed the most effective inhibitors against proteins 5XU1 and 4XCH into the order of ligand-protein complexes <strong>E12</strong>,<strong> E9</strong>,<strong> E5</strong>, <strong>E11</strong>,<strong> E8 </strong>and<strong> E8</strong>,<strong> E11</strong>,<strong> E9</strong>, <strong>E10</strong>,<strong> E12</strong>.</p> 2024-09-30T00:00:00+00:00 Copyright (c) 2024 Nguyen Vinh Phu, Vo Hoang Bach, Hoang Thi Phuong Nga, Tran Quang Huy, Nguyen Dai Chau, Nguyen Thi Ai Nhung, Phan Tu Quy, Nguyen Thi Thanh Hai*