Cloning gene encoding transcription factor DREB1 for regulating abiotic stress of L14 peanut variety
Vol. 132 No. 1C (2023), Original Article
Vol. 132 No. 1C (2023)

Cloning gene encoding transcription factor DREB1 for regulating abiotic stress of L14 peanut variety

Original Article
https://doi.org/10.26459/hueunijns.v132i1C.7077
Published 2023-09-30
Tran Linh Anh
Faculty of Biology, University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
Nguyen Quang Duc Tien*
Faculty of Biology, University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
PDF (Vietnamese)

Keywords

peanut variety L14
drought tolerance gene
cloning
DREB1 DREB1
tạo dòng
Giống lạc L14
stress phi sinh học

How to Cite

1.
Anh TL, Tiến NQ Đức. Cloning gene encoding transcription factor DREB1 for regulating abiotic stress of L14 peanut variety. hueuni-jns [Internet]. 2023Sep.30 [cited 2024Nov.15];132(1C):31-8. Available from: http://222.255.146.83/index.php/hujos-ns/article/view/7077
ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver

Download Citation

Endnote/Zotero/Mendeley (RIS) BibTeX
Crossref
Scopus
Google Scholar
Europe PMC

Abstract

Peanut (Arachis hypogaea L.) is an essential short-term industrial crop, producing most of the world's vegetable oil and protein. Peanuts are commonly used as human and animal food, as well as raw materials in a variety of industries. Peanuts are widely grown in tropical and subtropical regions, with Asia being the world's largest peanut-growing area, accounting for more than 67% of the total peanut production. Numerous studies have shown that identifying genes related to drought tolerance in plants is critical for the selection or improvement of drought-tolerant varieties to reduce the risk of damage caused by dry weather conditions. The transcription factor DREB (Dehydration-Responsive Element Binding Protein) has been shown to play a significant role in plant tolerance to drought stress responses. The gene encoding the transcription factor DREB1 in response to the drought-tolerant peanut variety L14 was isolated, sequenced, and analyzed in this study. The DREB1's cDNA sequence is 1050 nucleotides long and encodes 349 amino acids. The findings of the study reveal that the DREB1 gene in the L14 peanut variety differs from the published corresponding gene sequence from another peanut by 0.48%. The AhL14_DREB1 gene sequence is the basic information to improve the drought tolerance of local peanut varieties in further studies.

https://doi.org/10.26459/hueunijns.v132i1C.7077
PDF (Vietnamese)

References

  1. Zhang B, Su L, Hu B, Li L. Expression of AHDREB1, an AP2/ERF transcription factor gene from Peanut, is affected by histone acetylation and increases abscisic acid sensitivity and tolerance to osmotic stress in Arabidopsis. International Journal of Molecular Sciences. 2018;19(5):1441.
  2. Vien TD. Climate change and its impact on agriculture in Vietnam. Journal of the International Society for Southeast Asian Agricultural Sciences. 2011;17(1):17-21.
  3. Hou L, Liu W, Li Z, Huang C, Fang XL, Wang Q, Liu X. Identification and expression analysis of genes responsive to drought stress in Peanut. Russian Journal of Plant Physiology. 2014;61(6):842-852.
  4. Oanh PT, Thuỷ VTT, Tâm NT, Mậu CH. Sự sai khác về trình tự nucleotide trong gen cystatin ở một số dõng lạc có nguồn gốc từ mô sẹo của giống lạc L18. Tạp chí Khoa học & Công nghệ. 2009;85(09)1:133-141.
  5. Riechmann JL, Meyerowitz EM. The AP2/EREBP family of plant transcription factors. Biol Chem. 1998;379:633-646.
  6. Cui M, Haider MS, Chai P, Guo J, Du P, Li H, et al. Genome-Wide Identification and expression analysis of AP2/ERF transcription factor related to drought stress in cultivated Peanut (Arachis hypogaea L.). Frontiers in Genetics. 2021;12.
  7. Dang PM, Chen CY, Holbrook CC. Identification of drought-induced transcription factors in Peanut (Arachis hypogaea L.). Journal of Molecular Biochemistry. 2012;1(3).
  8. Ji AJ, Luo HM, Xu ZC, Zhang X, Zhu YJ, Liao BS, et al. Genome‐Wide Identification of the AP2/ERF gene family involved in active constituent biosynthesis in Salvia miltiorrhiza. The Plant Genome. 2016;9(2).
  9. Zhang M, Liu W, Bi Y, Wang Z. Isolation and identification of PNDREB1: a new DREB transcription factor from Peanut (Arachis hypogaea L.). Acta Agronomica Sinica. 2009;35(11):1973-1980.
  10. Park S, Shi A, Mou B. Genome-wide identification and expression analysis of the CBF/DREB1 gene family in Lettuce. Scientific reports. 2020;10(1):1-14.
  11. Li Z, Wang G, Liu X, Wang Z, Zhang M, Zhang J. Genome-wide identification and expression profiling of DREB genes in Saccharum spontaneum. BMC genomics. 2021;22(1):1-15.
  12. Latini A, Rasi C, Sperandei M, Cantale C, Iannetta M, Dettori M, et al. Identification of a DREB‐related gene in Triticum durum and its expression under water stress conditions. Annals of Applied Biology. 2007;150(2):187-195.
  13. Liu XQ, Zhu JJ, Wei CJ, Guo Q, Bian CK, Xiang ZH, et al. Genome-wide identification and characterization of the DREB transcription factor gene family in Mulberry. Biologia Plantarum. 2015;59(2):253-265.
  14. Yang W, Liu XD, Chi XJ, Wu CA, Li YZ, Song LL, et al. Dwarf apple MbDREB1 enhances plant tolerance to low temperature, drought, and salt stress via both ABA-dependent and ABA-independent pathways. Planta. 2011;233(2):219-229.
  15. Chai M, Cheng H, Yan M, Priyadarshani SVGN, Zhang M, He Q, et al. Identification and expression analysis of the DREB transcription factor family in Pineapple (Ananas comosus (L.) Merr.). PeerJ. 2020;8:e9006.
  16. Hassan S, Berk K, Aronsson H. Evolution and identification of DREB transcription factors in the wheat genome: modeling, docking and simulation of DREB proteins associated with salt stress. Journal of Biomolecular Structure and Dynamics. 2022;40(16):7191-7204.
  17. Niu X, Luo T, Zhao H, Su Y, Ji W, Li H. Identification of wheat DREB genes and functional characterization of TaDREB3 in response to abiotic stresses. Gene. 2020;740;144514.
  18. Tong Z, Hong B, Yang Y, Li Q, Ma N, Ma C, et al. Overexpression of two chrysanthemum DgDREB1 group genes causing delayed flowering or dwarfism in Arabidopsis. Plant Molecular Biology. 2009;71(1):115-129.
  19. Wang Q, Guan Y, Wu Y, Chen H, Chen F, Chu C. Overexpression of a rice OsDREB1F gene increases salt, drought, and low temperature tolerance in both Arabidopsis and rice. Plant Molecular Biology. 2008;67(6):589-602.
  20. Li X, Cheng X, Liu J, Zeng H, Han L, Tang W. Heterologous expression of the Arabidopsis DREB1A/CBF3 gene enhances drought and freezing tolerance in transgenic Lolium perenne plants. Plant Biotechnology Reports. 2011;5(1):61-69.
  21. James VA, Neibaur I, Altpeter F. Stress inducible expression of the DREB1A transcription factor from xeric, Hordeum spontaneum L. in turf and forage grass (Paspalum notatum Flugge) enhances abiotic stress tolerance. Transgenic Research. 2008;17(1):93-104.
  22. Chen JQ, Meng XP, Zhang Y, Xia M, Wang XP. Over-expression of OsDREB genes lead to enhanced drought tolerance in rice. Biotechnology letters. 2008;30(12):2191-2198.
  23. Gutha LR, Reddy AR. Rice DREB1B promoter shows distinct stress-specific responses, and the overexpression of cDNA in tobacco confers improved abiotic and biotic stress tolerance. Plant Molecular Biology. 2008;68(6):533-555.
  24. Yin D, Liu H, Zhang X, Cui D. A protocol for extraction of high-quality RNA and DNA from peanut plant tissues. Molecular biotechnology. 2011;49:187-191.
  25. Banjara M, Zhu L, Shen G, Payton P, Zhang H. Expression of an Arabidopsis sodium/proton antiporter gene (AtNHX1) in peanut to improve salt tolerance. Plant biotechnology reports. 2012;6:59-67.
Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Copyright (c) 2023 Array