Microbial preparation for pesticide residues (chlorpyrifos ethyl) treatment in agricultural soil
Vol. 134 No. 1S-1 (2025), Special Issue: IFGTM 2025
Vol. 134 No. 1S-1 (2025)

Microbial preparation for pesticide residues (chlorpyrifos ethyl) treatment in agricultural soil

Special Issue: IFGTM 2025
https://doi.org/10.26459/hueunijns.v134i1S-1.7853
Published 2025-08-12
Huu Thanh Luong
Institute of Agricultural Environment, Sa Đoi, Phu Do, Nam Tu liem, Ha Noi, Vietnam
Thuy Nga Vu
Institute of Agricultural Environment, Sa Đoi, Phu Do, Nam Tu liem, Ha Noi, Vietnam
Ngoc Quynh Nguyen
Institute of Agricultural Environment, Sa Đoi, Phu Do, Nam Tu liem, Ha Noi, Vietnam
Trong Anh Dam
Institute of Agricultural Environment, Sa Đoi, Phu Do, Nam Tu liem, Ha Noi, Vietnam
Thanh Lam Nguyen
Ministry of Agriculture and Environment, 10 Ton That Thuyet, Nam Tu Liem, Ha Noi, Vietnam
Thi Nhu Hang Tran
Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Vietnam
Nguyen Kieu Bang Tam*
Faculty of Environmental Sciences, University of Science, Vietnam National University, Ha Noi, Vietnam
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Keywords

pesticide
microbial preparation
chlorpyrifos ethyl
organophosphate
residue treatment

How to Cite

1.
Luong HT, Vu TN, Nguyen NQ, Dam TA, Nguyen TL, Tran TNH, Nguyen Kieu BT. Microbial preparation for pesticide residues (chlorpyrifos ethyl) treatment in agricultural soil. hueuni-jns [Internet]. 2025Aug.12 [cited 2025Oct.11];134(1S-1):71-8. Available from: http://222.255.146.83/index.php/hujos-ns/article/view/7853
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Abstract

Chlorpyrifos ethyl (CPF), a widely used organophosphate pesticide, poses significant environmental risks because of its excessive application beyond recommended levels. This study presents the development of a microbial preparation (MP) for the treatment of CPF residues in soil using indigenous strains Methylobacterium populi (CNN2) and Ensifer adhaerens (VNN3), which were selected on the basis of their demonstrated CPF-degrading capability in prior research. The objective was to identify optimal conditions for biomass production to formulate a stable and effective MP. Laboratory-scale experiments were conducted to evaluate biosafety and determine ideal parameters, including pH, temperature, fermentation media, and inoculum ratio for microbial growth. The selected strains met European biosafety standards, exhibited mutual compatibility, and showed no antagonism toward beneficial microorganisms. Optimal biomass production was achieved at 30 °C and pH 6.5–7.0, in an SX1 medium with a 3% inoculum ratio. The strains demonstrated impressive in vitro CPF degradation capabilities, achieving up to 81% and 95% degradation efficiency for CNN2 and VNN3, respectively, after three days of cultivation. The developed MP maintained high viability with cell counts of 10⁹ CFU/g after one month, 10⁸ CFU/g after six months, and 10⁷ CFU/g after twelve months. These results demonstrate the potential of native, eco-friendly microbial agents for effective bioremediation of pesticide-contaminated agricultural soils.

https://doi.org/10.26459/hueunijns.v134i1S-1.7853
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