KINETIC CATALYTIC SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF IRON IN BLOOD AND SERUM

Authors

  • Nguyễn Văn Ly Trường Đại học Khoa học

Abstract

Abstract: Iron in blood and serum of some adults are determined by reaction between sulphanilic acid (SA) and potassium periodate (KIO4) with the catalytic of Fe(III), in the presence of cetylpyridinium chloride (CPC) surfactant and 1,10 - phenanthroline (Phen) activator. The reaction was monitored spectrophotometrically by measuring the increase in absorbance of the reaction product at 310 nm. By this reaction in the range 10 - 40 µg/L can be determined, and limit of detection is 2.9 µg/L. In this paper, we applied to determine of iron in blood and serum, thereby evaluate accuracy of method. After treatment samples by two different ways, the iron content in blood and serum are 129.20 ± 9.72 (or 133.15 ± 9.85) and 1.32 ± 0.15 (or 1.42 ± 0.13), respectively. The recovery changes between 94.3 and 96.7% (or 95.7 – 104.7%) for serum and exhibits from 90.8 to 97.3% (or 96.7 – 103.3%).

Author Biography

Nguyễn Văn Ly, Trường Đại học Khoa học

Giang vien chinh, Tien si

References

REFERENCES

Nguyen Van Ly (2013), “ Determination of iron trace by catalytic kinetic spectrophotometry”, Journal of Science, Hue University, Number 9, vol.73, No.9, pp. 121 – 129.

Waseem A., Yaqoob M. and Nabi A. (2004), “Determination of iron in blood serum using flow injection with luminol chemiluminescence detection”, Original Research, Luminesence, 19: 333 – 338.

Harington C. R., (1941), “The micro – determination of potassium”, department of chemical pathology, University college hospital medical shcool, London, W.C.1, 546 – 550.

Olson A. D. and Hamlin W. B. (1969), “A new method for serum iron and total iron-binding capacity by atomic absorption spectrophotometry”, The Swedish hospital medical center, Seattle, Wash 98104. Vol.25, No.6.

Stoyanova A. (2005), “Determination of trace iron(III) by catalytic kinetic spectrophotometry with N, N – diphenylamine”, Trakia Journal of Sciences, 3(3), pp. 10-15.

Published

2014-11-21

Issue

Section

Khoa học Tự Nhiên