METHODS DETERMINATION OF NITROUS OXIDE EMISSIONS IN RICE FIELD BY GAS CHROMATOGRAPHY (GC)
Abstract
ABSTRACT
This study was undertaken to develop and validate an accurate, sensitive and reproducible Gas chromatographic (GC) method for the quantitation of nitrous oxide emission in rice field. The sample and the standard were eluted from Porapak Q (4m×2mm ID glass, 80/100 mesh), Hayesep (4m×2mm ID glass, 80/100 mesh) (Supelco, Sigma – Aldrich Co, Singapore). Using nitrogen as carrier gas at a flow rate of 40kPa. Each analysis required no longer than 11 min. The limit of quantitation was 0.03 ppmV. Recovery for different concentrations ranged from 98% to 100%. The calibration curves were obtained in the concentration range 0.125–1.5 ppmV. The results showed a close correlation coefficient (R2 = 0.9998) for analyte in the calibration range studied. The reproducibility RSDs ranged from 4.02%. The simplicity of this low-cost, rapid technique and its high specificity to nitrous oxide, even in the presence of other gas, demonstrate this GC method would be particularly suitable for the determination of nitrous oxide emissions in rice fields of VietNam.
References
REFERENCES
Trần Cao Sơn, Phạm Xuân Đà, Lê Thị Hồng Hảo, Nguyễn Thành Trung, 2010. Thẩm định phương pháp trong phân tích hóa học và vi sinh. Viện nghiên cứu an toàn vệ sinh thực phẩm quốc gia.
IPCC. Third Assessment Report--Climate change 2001.The Scientifi Basis: chapter4, Atmospheric Chemistry and Greenhouse Gases.
Miller J.C and Miller J. N,1988. Statistics for Analytical Chemistry. Ellis Horwood Limited.
Mosier AR, Halvorson AD, Reule CA, Liu XJ (2006) Net global warming potential and greenhouse gas intensity in irrigated cropping systems in Northeastern Colorado. J Environ Qual 35:1584– 1598.
Pandey S, Velasco L (2002) Economics of direct seeding in Asia: patterns of adoption and research priorities. In: Pandey S, Mortimer M, Wade L, Tuong TP, Lopes K, Hardy B (eds) Direct seeding: research strategies and opportunities. Inter-national Rice Research Institute, Los Banõs.
Qin Y, Liu S, Guo Y, Liu Q, Zou J (2010) Methane and nitrous oxide emissions from organic and conventional rice cropping systems in Southeast China. Biol Fertil Soils 46:825–834.
Robertson GP, Paul EA, Harwood RR (2000). Greenhouse gases in intensive agriculture: contributions of individual gases to the radiative forcing of the atmosphere. Science 289:1922 –1925
Smith P, Martino D, Cai Z et al (2008). Greenhouse gas mitigation in agriculture. Phil Trans R Soc B 363:789 –813.
Wassmann R, Neue HU, Ladha JK, Aulakh MS (2004). Mitigating greenhouse gas emissions from rice-wheat cropping systems in Asia. Environ Dev Sustain 6:65 –90.
