Classification of some commercial lingzhi (Ganoderma spp.) accessions in Vietnam by its-based DNA barcode
Vol. 128 No. 1E (2019), Special Issues - Conference
Vol. 128 No. 1E (2019)

Classification of some commercial lingzhi (Ganoderma spp.) accessions in Vietnam by its-based DNA barcode

Special Issues - Conference
https://doi.org/10.26459/hueuni-jns.v128i1E.5380
Published 2019-10-29
Hồ Viết Thế
Ho Chi Minh City University of Food Industry, 140 Le Trong Tan St., Tan Phu District, Ho Chi Minh City, Vietnam
Võ Thị Ngọc Hà
Ho Chi Minh City University of Food Industry, 140 Le Trong Tan St., Tan Phu District, Ho Chi Minh City, Vietnam
Lê Ngọc Giàu
Ho Chi Minh City University of Food Industry, 140 Le Trong Tan St., Tan Phu District, Ho Chi Minh City, Vietnam
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Keywords

molecular marker
genetic diversity

How to Cite

1.
Viết Thế H, Thị Ngọc Hà V, Ngọc Giàu L. Classification of some commercial lingzhi (Ganoderma spp.) accessions in Vietnam by its-based DNA barcode . hueuni-jns [Internet]. 2019Oct.29 [cited 2024Nov.14];128(1E):163-71. Available from: http://222.255.146.83/index.php/hujos-ns/article/view/5380
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Abstract

In Vietnam, lingzhi is mainly used for medicinal purposes with a high market value leading to the increasing production in recent years. However, the identification and classification of this fungus are inaccurate due mainly to morphological characteristics. In this study, the ITS-based DNA barcode was used to analyze the genetic composition of 10 commercial lingzhi genotypes collected from different regions across the country. The results show that there exists genetic diversity of 10 lingzhi samples. Based on Kimura 2-parameter model, the genetic distances among studied accessions range from 0.000 to 0.047. Seven of ten accessions were reclassified as G. lingzhi and three accessions were identified as G. lucidum. In addition, 19 nucleotide sites differed among fungal samples were also reported. There is a high potential of using ITS-based DNA barcode for the identification and classification as well as providing information on the genetic relationship between lingzhi varieties.

https://doi.org/10.26459/hueuni-jns.v128i1E.5380
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References

  1. Pawlik A, Janusz G, Debska I, Siwulski M, Frac M, Rogalski J. Genetic and metabolic intraspecific biodiversity of Ganoderma lucidum. Biomed Res Int. 2015: doi: 10.1155/2015/726149
  2. Ahmedand THM, Mohamed ZMA. Genetic diversity of mango (Mangifera indica L.) cultivars in Shendi Area. J Appl Sci. 2014;3(6):219-224.
  3. Guglielmo F, Gonthier P, Garbelotto M, Nicolotti G. PCR-based method for the identification of important wood rotting fungal taxa within Ganoderma, Inonotus s.l. and Phellinuss.l. FEMS Microbiol Lett. 2010;282:228-237.
  4. Singh SK, Yadav MC, Upadhyay RC, Kama S, Rai RD, Tewari RP. Molecular characterization of specialty mushroom germplasm of the National Mushroom Repository. Mushroom Res. 2003;12:67-78.
  5. Zheng L, Jia D, Fei X, Luo X, Yang Z. An assessment of the genetic diversity within Ganoderma strains with AFLP and ITS PCR-RFLP, Microbiol Res. 2009;164:312-321.
  6. Park YJ. Genetic diversity analysis of Ganoderma species and development of a specific marker for identification of medicinal mushroom Ganoderma lucidum. Afr J Microbiol Res. 2012;6(25):5417-5425.
  7. Hennicke F, Cheikh-Ali Z, Liebisch T, Macia-Vicent JG, Bode HB, Piepenbring M. Distinguishing commercially grown Ganoderma lucidum from Ganoderma lingzhi from Europe and East Asia on the basis of morphology, molecular phylogeny, and triterpenic acid profiles. Phytochemistry. 2016;127:29-37.
  8. Liao B, Chen X, Han J, Dan Y, Wang L, Jiao W et al. Identification of commercial Ganoderma (Lingzhi) species by ITS2 sequences., Chin Med. 2015;10(1),22. DOI 10.1186/s13020-015-0056-7
  9. Loyd AL, Richter BS, Jusino MA, Truong C, Smith ME, Blanchette RA et al. Identifying the “Mushroom of Immortality: Assessing the Ganoderma species composition in commercial Reishi products. Front Microbiol. 2018; 9:1-14.
  10. Mei Z, Yang L, Khan MA, Yang M, Wei C, Yang W et al. Genotyping of Ganoderma species by improved random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) analysis. Biochem Syst Ecol. 2004; 56:40-48.
  11. Le DHV, Tran DH. Biological characterization and yield of some trains of lingshi mushroom (Ganoderma licidum) in Thua Thien Hue province. J Res Dev. 2008;49:209-216.
  12. Ngo XN, Nguyen TBT, Le VV, Nguyen TL, Nguyen TT, Nguyen DQ. Morphological characterisitcs, Yield performance, and mdicial value of some lingzhi mushroom (Ganoderma lucidum) strains cultivated in Tam Dao, Vietnam. Viet J Agril Sci. 2019; 2(1):321-331.
  13. Pham BT, Nguyen MT. Effects of temperature and time on extraction of polysaccharide and tannins from red lingzhi (Ganoderma lucidum). Can Tho Univ J Sci. 2015;36:21-28.
  14. Cao Y, Wu SH, Dai YC. Species clarification of the prize medicinal Ganoderma mushroom “Lingzhi”. Fungal Divers. 2012; 56(1):49-62.
  15. Porebski S, Bailey LG.Baum BR. Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol Biol. 1997;15:8-15.
  16. White T, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH Sninski JJ, White TJ Editors. PCR-protocols a guide to methods and applications., San Diego: Academic press. 1990:315-322.
  17. Kress WJ, Wurdack KJ, Zimmer EA, Weigt LA, Janzen DH. Use of DNA barcodes to identify flowering plants, Proc Natl Acad Sci U S A. 2002;102(23):8369-8374.
  18. Wang A, Gopurenko D, Wu H, Lepschi B. Evaluation of six candidate DNA barcode loci for identification of five important invasive grasses in eastern Australia. Plos One. 2016;12(4):e0175338
  19. Chen SL, Yao H, Han JP, Liu C, Song JY, Shi LC et al. Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species. PLoS One. 2010;5:e8613.
  20. Kwon O, Par Y, Kim H, Kong W, Cho J, Lee C. (Taxonomic postion and species identity of the cultivated Yeongji “Ganoderma lucidum” in Korea. Mycobiology. 2015;44(1):1-6.
  21. Liu J, Moller M, Ga LM, Zhang DQ, Li DZ. DNA barcoding for the discrimination of Eurasian Yews (Taxus L., Taxaceae) and the discovery of cryptic species. Mol Ecol Resour. 2011;11:89-100.
  22. Ipek M, Ipek A, Simon WP. Testing the utility of matK and ITS DNA regions for discrimination of Allium species. Turk J Bot. 2014;28:203-212.
  23. Mishra P, Kumar A, Sivaraman G, Shukla AK, Kaliamoorthy R, Slater A Et al.Character-based DNA barcoding for authentication and conservation of IUCN Red listed threatened species of genus Decalepis (Apocynaceae). Sci Rep. 2017;7:14910.
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