Effect of extraction temperature on content and in vitro antioxidant activity of polysaccharide from Gymnopetalum cochinchinense
Vol. 134 No. 1A (2025), Original Article
Vol. 134 No. 1A (2025)

Effect of extraction temperature on content and in vitro antioxidant activity of polysaccharide from Gymnopetalum cochinchinense

Original Article
https://doi.org/10.26459/hueunijns.v134i1A.7491
Published 2025-03-19
Lan Huong Hoang Thi
Faculty of Chemistry, University of Sciences, Hue University, Hue, Vietnam
Thi Van Thi Tran
Faculty of Chemistry, University of Sciences, Hue University, Hue, Vietnam
Son Le Lam
Faculty of Chemistry, University of Sciences, Hue University, Hue, Vietnam
Chien Nguyen Chinh
Duy Tan University, Da Nang, Vietnam
Thuy Van Do Thi
University of Education, University of Danang, Da Nang, Vietnam
Minh Tran Thanh
Faculty of Chemistry, University of Sciences, Hue University, Hue, Vietnam
Thang Nguyen Viet
Faculty of Chemistry, University of Sciences, Hue University, Hue, Vietnam
Minh Tran Nhat
Hue Medical College, Hue, Vietnam
Thu Phuong Nguyen Thi
Thuan Hoa High School, University of Education, Hue University
Nhu Nguyen Thi
Faculty of Chemistry, University of Sciences, Hue University, Hue, Vietnam
Hong Hanh Nguyen Thi
Faculty of Chemistry, University of Sciences, Hue University, Hue, Vietnam
Le Trung Hieu*
Faculty of Chemistry, University of Sciences, Hue University, Hue, Vietnam
PDF (Vietnamese)

Keywords

Gymnopetalum cochinchinense
polysaccharide
antioxidant activities
DPPH
ABTS dây cứt quạ
polysaccharide
chống oxy hoá
DPPH
ABTS

How to Cite

1.
Hoàng Thị LH, Trần TVT, Lê Lâm S, Nguyễn Chinh C, Đỗ Thị TV, Trần Thanh M, Nguyễn Việt T, Trần Nhật M, Nguyễn Thị TP, Nguyễn Thị N, Nguyễn Thị HH, Trung Hiếu L. Effect of extraction temperature on content and in vitro antioxidant activity of polysaccharide from Gymnopetalum cochinchinense. hueuni-jns [Internet]. 2025Mar.19 [cited 2025Apr.26];134(1A):143-50. Available from: http://222.255.146.83/index.php/hujos-ns/article/view/7491
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Abstract

The effect of extraction temperature on the in vitro antioxidant activity of polysaccharides from Gymnopetalum cochinchinense was investigated. The results show that at an extraction temperature of 80 °C, the antioxidant activity of the polysaccharides was the highest. The total antioxidant capacity of the PS-W-T80 sample was 0.2726 ± 0.0003 mg GA/g or 0.1776 ± 0.0001 mg AS/g. Furthermore, its IC50 values in the DPPH and ABTS radical scavenging models were 0.83 and 3.30 mg/mL, respectively. Fourier transform infrared spectroscopy analysis indicates that the polysaccharides in the PS-W-T80 sample exhibit characteristic absorption peaks commonly found in polysaccharide structures. The average molecular weight of the extracted polysaccharide is approximately 5.56 × 104 Da. These results show that the polysaccharide in PS-W-T80 from G. cochinchinense is a potential antioxidant agent.

https://doi.org/10.26459/hueunijns.v134i1A.7491
PDF (Vietnamese)

References

  1. Zong A, Cao H, Wang F. Anticancer polysaccharides from natural resources: A review of recent research. Carbohydrate polymers. 2012;90(4):1395-410.
  2. Khan T, Date A, Chawda H, Patel K. Polysaccharides as potential anticancer agents—A review of their progress. Carbohydrate polymers. 2019;210:412-28.
  3. Li C, Dong Z, Zhang B, Huang Q, Liu G, Fu X. Structural characterization and immune enhancement activity of a novel polysaccharide from Moringa oleifera leaves. Carbohydrate polymers. 2020;234:115897.
  4. Hou C, Chen L, Yang L, Ji X. An insight into anti-inflammatory effects of natural polysaccharides. International journal of biological macromolecules. 2020;153:248-55.
  5. De Wilde W, Duyfjes B. Review of the genus Gymnopetalum (Cucurbitaceae). Blumea-Biodiversity, Evolution and Biogeography of Plants. 2006;51(2):281-96.
  6. Kumar S, Das G, Shin H-S, Kumar P, Patra JK. Evaluation of medicinal values of Gymnopetalum chinense (Lour.) Merr., a lesser known cucurbit from Eastern Ghats of India. Brazilian Archives of Biology and Technology. 2017;60:e17160580.
  7. Tapan S, Kaushik C, Basundhara P. Antioxidant activity of some selected wild edible fruits of North-eastern region in India and effect of solvent extraction system. Global Journal of Environmental Research. 2012;6(3):84-90.
  8. Rahmatullah M. A survey of preventive medicinal plants used by the Chakma residents of Hatimara (south) village of Rangamati district, Bangladesh. Am-Eur J Sustainable Agric. 2011;5:92-6.
  9. Cordenunsi BR, Genovese MI, do Nascimento JRO, Hassimotto NMA, dos Santos RJ, Lajolo FM. Effects of temperature on the chemical composition and antioxidant activity of three strawberry cultivars. Food chemistry. 2005;91(1):113-21.
  10. Kan Y, Chen T, Wu Y, Wu J. Antioxidant activity of polysaccharide extracted from Ganoderma lucidum using response surface methodology. International journal of biological macromolecules. 2015;72:151-7.
  11. Tan X, Sun J, Xu Z, Li H, Hu J, Ning H, et al. Effect of heat stress on production and in-vitro antioxidant activity of polysaccharides in Ganoderma lucidum. Bioprocess and biosystems engineering. 2018;41:135-41.
  12. DuBois M, Gilles KA, Hamilton JK, Rebers Pt, Smith F. Colorimetric method for determination of sugars and related substances. Analytical chemistry. 1956;28(3):350-6.
  13. Nair VD, Panneerselvam R, Gopi R. Studies on methanolic extract of Rauvolfia species from Southern Western Ghats of India–In vitro antioxidant properties, characterisation of nutrients and phytochemicals. Industrial Crops and Products. 2012;39:17-25.
  14. Le TH, Nguyen THC, Le LS, Nguyen DGC, Tran TVT, Nguyen MN, et al. Unfolding the structure of polysaccharide isolated from Myxopyrum smilacifolium leaves toward a robust antioxidant. Bioactive Carbohydrates and Dietary Fibre. 2023;29:100347.
  15. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine. 1999;26(9-10):1231-7.
  16. Long X, Yan Q, Peng L, Liu X, Luo X. Effect of various temperatures on bletillae rhizoma polysaccharide extraction and physicochemical properties. Applied Sciences. 2018;9(1):116.
  17. Khoa TV, Vu HXA, Kiet TT, Van Thi TT. Effect of extraction temperatures on in vitro antioxidant activities of polysaccharides from Ophiocordyceps sobolifera. Hue University Journal of Science: Natural Science. 2019;128(1D):17-21.
  18. Chen Y, Xie MY, Nie SP, Li C, Wang YX. Purification, composition analysis and antioxidant activity of a polysaccharide from the fruiting bodies of Ganoderma atrum. Food Chemistry. 2008;107(1),231-241.
  19. Zhang J, Wen C, Chen M, Gu J, Zhou J, Duan Y, et al. Antioxidant activities of Sagittaria sagittifolia L. polysaccharides with subcritical water extraction. International journal of biological macromolecules. 2019;134:172-9.
  20. Li C, Huang Q, Fu X, Yue X-J, Liu RH, You L-J. Characterization, antioxidant and immunomodulatory activities of polysaccharides from Prunella vulgaris Linn. International Journal of Biological Macromolecules. 2015;75:298-305.
  21. Le TH, Thi Tran TV, Tran VK, Vu Ho XA, Tran TM, Chau Nguyen DG, et al. Structural characterization of mannoglucan isolated from Ophiocordyceps sobolifera and its antioxidant activities. ACS omega. 2022;7(11):9397-405.
  22. Chen W, Liu G, Yang H, Zhou H, Yang H. Effects of processing treatments on the antioxidant properties of polysaccharide from Cordyceps militaris. International Journal of Food Engineering. 2017;13(1):20160076.
  23. Karnchanatat A, Puthong S, Sihanonth P, Piapukiew J, Sangvanich P. Antioxidation and antiproliferation properties of polysaccharide-protein complex extracted from Phaeogyroporus portentosus (Berk. Broome) McNabb. Afr J Microbiol Res. 2013;7:1668-80.
  24. Tran TVT, Ho XAV, Le LS, Le TH, Nguyen THC, Nguyen CC, et al. Chemical structure of a novel heteroglycan polysaccharide isolated from the biomass of Ophiocordyceps Sobolifera. Journal of Molecular Structure. 2021;1232:129986.
  25. Kacurakova M, Capek P, Sasinkova V, Wellner N, Ebringerova A. FT-IR study of plant cell wall model compounds: pectic polysaccharides and hemicelluloses. Carbohydrate polymers. 2000;43(2):195-203.
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