Simultaneous detection of paracetamol, chlorpheniramine maleate, cinnarizine, loratadine, and cetirizine hydrochloride in preparations derived from medicinal herbs by using high-performance liquid chromatography
Vol. 134 No. 1A (2025), Original Article
Vol. 134 No. 1A (2025)

Simultaneous detection of paracetamol, chlorpheniramine maleate, cinnarizine, loratadine, and cetirizine hydrochloride in preparations derived from medicinal herbs by using high-performance liquid chromatography

Original Article
https://doi.org/10.26459/hueunijns.v134i1A.7601
Published 2025-03-19
Hai Phong Nguyen
Faculty of Chemistry, University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
Thi Cam Minh Dao
Faculty of Pharmacy, University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen St, Hue, Viet Nam
Thi Thu Thuy Tran
Faculty of Pharmacy, University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen St, Hue, Viet Nam
Huynh Van Chung*
Faculty of Pharmacy, Buon Ma Thuot Medical University, 298 Ha Huy Tap St., Đak Lak, Viet Nam
PDF (Vietnamese)

How to Cite

1.
Nguyễn HP, Đào TCM, Trần TTT, Huỳnh VC. Simultaneous detection of paracetamol, chlorpheniramine maleate, cinnarizine, loratadine, and cetirizine hydrochloride in preparations derived from medicinal herbs by using high-performance liquid chromatography. hueuni-jns [Internet]. 2025Mar.19 [cited 2025Apr.26];134(1A):89-101. Available from: http://222.255.146.83/index.php/hujos-ns/article/view/7601
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Abstract

This study was conducted to develop a method for simultaneous quantification of paracetamol (PAR), chlorpheniramine maleate (CPM), cinnarizine (CET), loratadine (LOR), and cetirizine hydrochloride (CIN) in preparations derived from medicinal herbs by using high-performance liquid chromatography (HPLC) with a PDA detector. The analytical method was performed under the following chromatographic conditions: InertSustainTM C18 column (250 mm × 4.6 mm, 5 μm); mobile phase: a mixture of ACN (A) and 5 mM NaH2PO4 (pH = 2.3) (B) according to a gradient program; flow rate: 1.0 mL/min; sample injection volume: 20 μL; column temperature: 40 °C; detection wavelength: 245 nm for Paracetamol, 225 nm for chlorpheniramine maleate, 230 nm for cetirizine hydrochloride, 254 nm for loratadine, and 253 nm for cinarizin. The analytical method was validated according to ICH and AOAC guidelines. The detection limits of PAR, CPM, CET, LOR, and CIN were 2.00, 2.00, 1.75, 1.00, and 0.50 µg/mL, respectively. The developed procedure was applied to analyze 32 samples of traditional medicinal products collected from the local markets.

https://doi.org/10.26459/hueunijns.v134i1A.7601
PDF (Vietnamese)

References

  1. Roman Stanaszek WL. Pharmaceutical preparations in illicit drug cases. Problems of Forensic Sciences. 2001;XLVI:152-7.
  2. HU QC, Yi-ling; WANG, Ke; JI, Shen. Detection of 16 anti-inflammatory and anti-histamine chemical drugs added illegally into traditional Chinese medicine and health food by liquid chromatography-ion trap mass spectrometry method Chinese Journal of Pharmaceutical Analysis. 2008;28(12):2065-8.
  3. Miller GM, Stripp R. A study of western pharmaceuticals contained within samples of Chinese herbal/patent medicines collected from New York City’s Chinatown. Legal Medicine. 2007;9(5):258-64.
  4. Nili-Ahmadabadi A, Borzouee Z, Ahmadimoghaddam D, Firozian F, Dastan D. The occurrence of acetaminophen/codeine as an adulterant in herbal analgesic supplements in Hamadan, Iran: A pilot study. Complement Ther Med. 2019;42:223-5.
  5. Kim JY, Choi JY, Yoon CY, Cho S, Kim WS, Do JA. LC–MS/MS monitoring of 22 illegal antihistamine compounds in health food products from the Korean market. Journal of the Korean Society for Applied Biological Chemistry. 2015;58(1):137-47.
  6. Bộ Y tế. Dược thư quốc gia Việt Nam. Hà Nội: Nhà xuất bản Y học Hà Nội; 2007.
  7. Bộ Y tế. Thông tư số 10/2021/TT-BYT ngày 30/06/2021 về “Quy định danh mục chất cấm sử dụng trong sản xuất, kinh doanh thực phẩm bảo vệ sức khỏe"; Hà Nội: Bộ Y tế; 2021.
  8. Tuyền PTT, An NT, Hải ĐTT, Hạnh TT, Anh NTK. Nghiên cứu phát hiện các thuốc chống dị ứng trộn trái phép trong chế phẩm đông dược bằng LC-MS/MS. Tạp chí Dược học. 2018;3(517):70-4.
  9. Bogusz MJ, Hassan H, Al-Enazi E, Ibrahim Z, Al-Tufail M. Application of LC–ESI–MS–MS for detection of synthetic adulterants in herbal remedies. Journal of Pharmaceutical and Biomedical Analysis. 2006;41(2):554-64.
  10. Kim EH, Seo HS, Ki NY, Park N-H, Lee W, Do JA, et al. Reliable screening and confirmation of 156 multi-class illegal adulterants in dietary supplements based on extracted common ion chromatograms by ultra-high-performance liquid chromatography-quadrupole/time of flight-mass spectrometry. Journal of Chromatography A. 2017;1491:43-56.
  11. Lin Y-P, Lee Y-L, Hung C-Y, Chang C-F, Chen Y. Detection of adulterated drugs in traditional Chinese medicine and dietary supplements using hydrogen as a carrier gas. PLOS ONE. 2018;13(10):e0205371.
  12. Song N, Zhang K, Liu X, Sang T, Sun Y, Teng N. Determination of twelve chemical drugs illegally added in herbal tea by ultra highperformance liquid chromatography-tandem mass spectrometry coupled with modified QuEChERS]. Se Pu. 2015;33(10):1026-31.
  13. Khoshayand MR, Abdollahi H, Ghaffari A, Shariatpanahi M, Farzanegan H. Simultaneous spectrophotometric determination of paracetamol, phenylephrine and chlropheniramine in pharmaceuticals using chemometric approaches. Daru. 2010;18(4):292-7.
  14. Chawe LCD, Tittikpina NK, Ndiaye SM, Diop A, Ndiaye B, Fall D, et al. Validation of an UV-Visible spectrophotometry assay method for the determination of chlorpheniramine maleate tablets without prior extraction. International Journal of Biological and Chemical Sciences. 2021;15(1).
  15. Raad A, Al-healy F. UV-Spectral Studies on Chlorpheniramine Maleate In Pure Form And Pharmaceutical Preparations. Egyptian Journal of Chemistry. 2021;64:4151-6.
  16. Liu S-Y, Woo S-O, Koh H-L. HPLC and GC–MS screening of Chinese proprietary medicine for undeclared therapeutic substances. Journal of Pharmaceutical and Biomedical Analysis. 2001;24(5):983-92.
  17. Kim JY, Do JA, Choi JY, Cho S, Kim WS, Yoon CY. Development and validation of an ultra-performance liquid chromatography method for simultaneous analysis of 20 antihistaminics in dietary supplements. Biomed Chromatogr. 2015;29(3):465–74.
  18. AOAC. Guidelines for Standard Method Performance Requirements. In: Appendix F, editor; 2016. p. 1–18.
  19. ICH. Validation of analytical procedures: Text and Methodology; 2005. p. 1–17.
  20. Lan TT, Quỳnh NT, Anh NTK. Nghiên cứu xác định một số thuốc kháng histamin H1 trộn trái phép trong chế phẩm Đông dược bằng HPLC-PDA. Tạp chí dược học. 2020;258:35-40.
  21. Chittrakarn S, Penjamras P, Keawpradub N. Quantitative analysis of mitragynine, codeine, caffeine, chlorpheniramine and phenylephrine in a kratom (Mitragyna speciosa Korth.) cocktail using high-performance liquid chromatography. Forensic Sci Int. 2012;217(1–3):81-6.
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