trnL-trnF gene region analysis of natural Curuligio orchioides collected in Thua Thien Hue
Vol. 132 No. 1A (2023), Original Article
Vol. 132 No. 1A (2023)

trnL-trnF gene region analysis of natural Curuligio orchioides collected in Thua Thien Hue

Original Article
https://doi.org/10.26459/hueunijns.v132i1A.6958
Published 2023-03-31
Bui Le Thanh Nhan*
University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen St., Hue, Vietnam
Truong Thi Bich Phuong
University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
Hoang Tan Quang
Institute of Biotechnology, Hue University, Hue, Vietnam
PDF (Vietnamese)

Keywords

Sâm cau
đa dạng di truyền
trnF
trnL Curculigo orchioides
genetic diversity

How to Cite

1.
Bùi LTN, Trương TBP, Hoàng TQ. trnL-trnF gene region analysis of natural Curuligio orchioides collected in Thua Thien Hue. hueuni-jns [Internet]. 2023Mar.31 [cited 2024Nov.15];132(1A):75-82. Available from: http://222.255.146.83/index.php/hujos-ns/article/view/6958
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Abstract

Curculigo orchioides, a member of the genus Curculigo and the family Hypoxidaceae, is a herbal plant and has been used as traditional medicine. Currently, there is high demand for cultivating this plant. However, there has not been sufficient research into the genetic diversity to assist the authentication of the material, for example, the genetic research based on the DNA indicators of Curculigo. In this study, the gene trnL-trnF region of 15 Curculigo orchioides germplasms collected from three locations in the province were sequenced and analyzed for identification and evolution. The results show that the sequenced fragment of the gene trnL-trnF from the surveyed samples has 99% similarity and 100% coverage compared with the NC 053892 sequence of Curculigo orchioides. We identified five haplotypes of C. orchioides in the province with a haplotype diversity coefficient (Hd) of 0.705 ± 0.088. The genetic distances ranged from 0 to 0.49% among the populations. The Curculigo orchioides population in Thua Thien Hue has evolved towards balanced, neutral, and random selection because of the lack of rare alleles.

https://doi.org/10.26459/hueunijns.v132i1A.6958
PDF (Vietnamese)

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