Establishment of culture conditions for peripheral blood mononuclear cells from dogs (Canis lupus familiaris) for in vitro immunological modeling and screening
Vol. 135 No. 1S-1 (2026), Special Issue: IFB 2025
Vol. 135 No. 1S-1 (2026)

Establishment of culture conditions for peripheral blood mononuclear cells from dogs (Canis lupus familiaris) for in vitro immunological modeling and screening

Special Issue: IFB 2025
https://doi.org/10.26459/hueunijns.v135i1S-1.8057
Published 2026-10-12
Nguyen Trung Khang
Stem Cell Laboratory, Institute of Food and Biotechnology, Can Tho University, Cantho, Vietnam
Nguyen Thanh Lam
Faculty of Veterinary Medicine, College of Agriculture, Can Tho University, Cantho, Vietnam
Tran Thi Thanh Khuong*
Stem Cell Laboratory, Institute of Food and Biotechnology, Can Tho University, Cantho, Vietnam
PDF (Vietnamese)

Keywords

cell culture
dog
FBS
MTT
peripheral blood mononuclear cells tế bào đơn nhân máu ngoại vi
chó
nuôi cấy tế bào
FBS
MTT

How to Cite

1.
Khang NT, Lãm NT, Khương TTT. Establishment of culture conditions for peripheral blood mononuclear cells from dogs (Canis lupus familiaris) for in vitro immunological modeling and screening. hueuni-jns [Internet]. 2026Oct.12 [cited 2026Oct.29];135(1S-1):159-71. Available from: http://222.255.146.83/index.php/hujos-ns/article/view/8057
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Abstract

This study aimed to establish culture conditions for peripheral blood mononuclear cells (PBMCs) from dogs (Canis lupus familiaris) for application in immunological models and in vitro testing. PBMCs were isolated from whole blood using Ficoll gradient centrifugation and then cultured in 96-well plates using two different media (RPMI-1640 and DMEM) supplemented with three concentrations of fetal bovine serum (FBS: 5%, 10%, 15%). Parameters including cell morphology, cell density, viability of suspended cells, and adhesion cell viability (assessed by the MTT assay) were evaluated at days 1, 3, 6, and 9. The results showed that RPMI-1640 medium maintained a higher density of suspended cells, particularly on day 6 (2.733 ± 0.104 ×10⁵ cells/mL; p < 0.05), while DMEM supplemented with 10% FBS promoted better cell adhesion and sustained higher MTT optical density (OD) values, reflecting the presence of more viable cells with active intracellular metabolic enzyme activity. There was no significant difference in viability or MTT OD between the 10% and 15% FBS groups, indicating that 10% FBS is an appropriate concentration for culture applications.

https://doi.org/10.26459/hueunijns.v135i1S-1.8057
PDF (Vietnamese)

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