Antimicrobial activity against Vibrio spp. and probiotic potential of Lactobacillus farciminis HN12

Abstract

In this study, a lactic acid bacterium that is able to inhibit V. parahaemolyticus was isolated from fermented food “mam nem”. The nucleotide sequence of 16S rRNA comparison with the Genbank database indicates that the isolate exhibits high similarity with Lactobacillus farciminis and is named L. farciminis HN12. The isolate displays strong activity against V. parahaemolyticus at 460 AU/mL and inhibits the growth of 20 Vibrio spp. strains isolated from infected shrimp and fish with antibacterial activity ranging from 160 to 400 AU/mL. This strain also shows the ability to survive under stress conditions, such as the presence of bile salts and high NaCl concentrations. Its autoaggregation activity is 34.88%, and its coaggregation activity with E. coli is 59.05%. The isolate is inhibited by ampicillin and chloramphenicol. L. farciminis HN12 exhibits good antibacterial properties and has the properties of beneficial bacteria, which can potentially be used as probiotics for the aquaculture industry.